Construct: ORF TRCN0000471863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009291.1_s317c1
- Derived from:
- ccsbBroadEn_00282
- DNA Barcode:
- CCCTGGGACGAGCCCTTTAGTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKN1A (1026)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471863
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1026 | CDKN1A | cyclin dependent kinase inh... | NM_000389.5 | 100% | 100% | |
| 2 | human | 1026 | CDKN1A | cyclin dependent kinase inh... | NM_001220777.1 | 100% | 100% | |
| 3 | human | 1026 | CDKN1A | cyclin dependent kinase inh... | NM_001220778.1 | 100% | 100% | |
| 4 | human | 1026 | CDKN1A | cyclin dependent kinase inh... | NM_078467.2 | 100% | 100% | |
| 5 | human | 1026 | CDKN1A | cyclin dependent kinase inh... | NM_001291549.2 | 82.8% | 82.8% | 1_102del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agaaccggct ggggatgtcc gtcagaaccc atgcggcagc aaggcctgcc 121 gccgcctctt cggcccagtg gacagcgagc agctgagccg cgactgtgat gcgctaatgg 181 cgggctgcat ccaggaggcc cgtgagcgat ggaacttcGA CTTTGTCACC GAGACACCAC 241 TGGAGGGTGA CTTCGCCTGG GAGCGTGTGC GGGGCCTTGG CCTGCCCAAG CTCTACCTTC 301 CCACGGGGCC CCGGCGAGGC CGGGATGAGT TGGGAGGAGG CAGGCGGCCT GGCACCTCAC 361 CTGCTCTGCT GCAGGGGACA GCAGAGGAAG ACCATGTGGA CCTGTCACTG TCTTGTACCC 421 TTGTGCCTCG CTCAGGGGAG CAGGCTGAAG GGTCCCCAGG TGGACCTGGA GACTCTCAGG 481 GTCGAAAACG GCGGCAGACC AGCATGACAG ATTTCTACCA CTCCAAACGC CGGCTGATCT 541 TCTCCAAGAG GAAGCCCTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCCTGGG ACGAGCCCTT 721 TAGTCATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt