Construct: ORF TRCN0000471868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003798.1_s317c1
- Derived from:
- ccsbBroadEn_06158
- DNA Barcode:
- GTAACCAGCGCCCTAAATCCACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EMP3 (2014)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471868
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2014 | EMP3 | epithelial membrane protein 3 | NM_001313905.1 | 99.7% | 100% | 360T>C |
| 2 | human | 2014 | EMP3 | epithelial membrane protein 3 | NM_001425.3 | 99.7% | 100% | 360T>C |
| 3 | human | 2014 | EMP3 | epithelial membrane protein 3 | XM_011526605.3 | 99.7% | 100% | 360T>C |
| 4 | mouse | 13732 | Emp3 | epithelial membrane protein 3 | NM_001146346.1 | 87.1% | 92% | (many diffs) |
| 5 | mouse | 13732 | Emp3 | epithelial membrane protein 3 | NM_010129.2 | 87.1% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc actcctcttg ctggtggtct cagcccttca catcctcatt cttatactgc 121 ttttcgtggc cactttggac aagtcctggt ggactctccc tgggaaagag tccctgaatc 181 tctggtacga ctgcacgtgg aacaacgaca ccaaaacatg ggccTGCAGT AATGTCAGCG 241 AGAATGGCTG GCTGAAGGCG GTGCAGGTCC TCATGGTGCT CTCCCTCATT CTCTGCTGTC 301 TCTCCTTCAT CCTGTTCATG TTCCAGCTCT ACACCATGCG ACGAGGAGGT CTCTTCTATG 361 CCACCGGCCT CTGCCAGCTT TGCACCAGCG TGGCGGTGTT TACTGGCGCC TTGATCTATG 421 CCATCCACGC CGAGGAGATC CTGGAGAAGC ACCCGCGAGG GGGCAGCTTC GGATACTGCT 481 TCGCCCTGGC CTGGGTGGCC TTCCCCCTCG CCCTGGTCAG CGGCATCATC TACATCCACC 541 TACGGAAGCG GGAGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GTAACCAGCG CCCTAAATCC 721 ACATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt