Transcript: Human NM_001425.3

Homo sapiens epithelial membrane protein 3 (EMP3), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
EMP3 (2014)
Length:
649
CDS:
75..566

Additional Resources:

NCBI RefSeq record:
NM_001425.3
NBCI Gene record:
EMP3 (2014)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001425.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000116050 ACGTGGAACAACGACACCAAA pLKO.1 204 CDS 100% 4.950 6.930 N EMP3 n/a
2 TRCN0000116051 CCTTCACATCCTCATTCTTAT pLKO.1 104 CDS 100% 13.200 9.240 N EMP3 n/a
3 TRCN0000289890 CCTTCACATCCTCATTCTTAT pLKO_005 104 CDS 100% 13.200 9.240 N EMP3 n/a
4 TRCN0000308225 CTGGGAAAGAGTCCCTGAATC pLKO_005 169 CDS 100% 10.800 7.560 N EMP3 n/a
5 TRCN0000308230 GACGAGGAGGTCTCTTCTATG pLKO_005 349 CDS 100% 10.800 7.560 N EMP3 n/a
6 TRCN0000308224 TCATGGTGCTCTCCCTCATTC pLKO_005 280 CDS 100% 10.800 7.560 N EMP3 n/a
7 TRCN0000116047 CCCTTCACATCCTCATTCTTA pLKO.1 103 CDS 100% 5.625 3.938 N EMP3 n/a
8 TRCN0000116049 CGCCTTGATCTATGCCATTCA pLKO.1 416 CDS 100% 4.950 3.465 N EMP3 n/a
9 TRCN0000289965 CGCCTTGATCTATGCCATTCA pLKO_005 416 CDS 100% 4.950 3.465 N EMP3 n/a
10 TRCN0000116048 TGCAGTAATGTCAGCGAGAAT pLKO.1 234 CDS 100% 4.950 3.465 N EMP3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001425.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06158 pDONR223 100% 99.7% 100% None 360T>C n/a
2 ccsbBroad304_06158 pLX_304 0% 99.7% 100% V5 360T>C n/a
3 TRCN0000471868 GTAACCAGCGCCCTAAATCCACAT pLX_317 66.9% 99.7% 100% V5 360T>C n/a
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