Construct: ORF TRCN0000471874
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007117.1_s317c1
- Derived from:
- ccsbBroadEn_13962
- DNA Barcode:
- ACTTTCACCTTCACGATGCCTCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SULT2B1 (6820)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471874
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6820 | SULT2B1 | sulfotransferase family 2B ... | NM_177973.2 | 99.8% | 98.9% | 834C>T;1085_1086insA |
| 2 | human | 6820 | SULT2B1 | sulfotransferase family 2B ... | NM_004605.2 | 95.2% | 90.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1164
- ORF length:
- 1098
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgggcccgcc gagccccaga tcccgggctt gtgggacacc tatgaagatg 121 acatctcgga aatcagccag aagttgccag gtgaatactt ccggtacaag ggcgtcccct 181 tccccgtcgg cctgtactcg ctcgagagca tcagcttggc ggagaacacc caagatgtgc 241 gggacgacga catctttatc atcacctacc ccaagtcagg cacgacctgg atgatcgaga 301 tcatctgctt aatcctgaag gaaggggatc catcctggat ccgctccgtg cccatctggg 361 agcgggcacc ctggtgtgag accattgtgg gtgccttcag cctcccggac cagtacagcc 421 cccgcctcat gagctcccat cttcccatcc agatcttcac caaggccttc ttcagctcca 481 aggccaaggt gatctacatg ggccgcaacc cccgggacgt tgtggtctcc ctctatcatt 541 actccaagat cgccgggcag ttaaaggacc cgggcacacc cgaccagttc ctgagggact 601 tcctcaaagg cgaagtgcag tttggctcct ggttcgacca cattaagggc tggcttcgga 661 tgaagggcaa agacaacttc ctatttatca cctacgagga gctgcagcag gacttacagg 721 gctccgtgga gcgcatctgt gggttcctgg gccgtccgct gggcaaggag gcactgggct 781 ccgtcgtggc acactcaacc ttcagcgcca tgaaggccaa caccatgtcc aactacacgc 841 tgctgcctcc cagcctgcTG GACCACCGTC GCGGGGCCTT CCTCCGGAAA GGGGTCTGTG 901 GCGACTGGAA GAACCACTTC ACGGTGGCCC AGAGCGAAGC CTTCGATCGT GCCTACCGCA 961 AGCAGATGCG GGGGATGCCG ACCTTCCCCT GGGATGAAGA CCCGGAGGAG GACGGCAGCC 1021 CAGATCCTGA GCCCAGCCCT GAGCCTGAGC CCAAGCCCAG CCTTGAGCCC AACACCAGCC 1081 TGGAGCGTGA GCCCAGACCC AACTCCAGCC CCAGCCCCAG CCCCGGCCAG GCCTCTGAGA 1141 CCCCGCACCC AACGACCCTC ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 1201 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 1261 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACT TTCACCTTCA 1321 CGATGCCTCT TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 1381 t