Construct: ORF TRCN0000471878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003198.1_s317c1
- Derived from:
- ccsbBroadEn_02482
- DNA Barcode:
- GTGTCGGGTTTGCTATCGAGTCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDC42EP3 (10602)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10602 | CDC42EP3 | CDC42 effector protein 3 | NM_001270436.2 | 99.7% | 99.6% | 297_298delCTinsTC |
| 2 | human | 10602 | CDC42EP3 | CDC42 effector protein 3 | NM_001270437.2 | 99.7% | 99.6% | 297_298delCTinsTC |
| 3 | human | 10602 | CDC42EP3 | CDC42 effector protein 3 | NM_001270438.2 | 99.7% | 99.6% | 297_298delCTinsTC |
| 4 | human | 10602 | CDC42EP3 | CDC42 effector protein 3 | NM_001371569.1 | 99.7% | 99.6% | 297_298delCTinsTC |
| 5 | human | 10602 | CDC42EP3 | CDC42 effector protein 3 | NM_001371570.1 | 99.7% | 99.6% | 297_298delCTinsTC |
| 6 | human | 10602 | CDC42EP3 | CDC42 effector protein 3 | NM_006449.5 | 99.7% | 99.6% | 297_298delCTinsTC |
| 7 | human | 10602 | CDC42EP3 | CDC42 effector protein 3 | XM_011532482.3 | 99.7% | 99.6% | 297_298delCTinsTC |
| 8 | mouse | 260409 | Cdc42ep3 | CDC42 effector protein (Rho... | XM_006524295.3 | 87.5% | 92.1% | (many diffs) |
| 9 | mouse | 260409 | Cdc42ep3 | CDC42 effector protein (Rho... | NM_026514.2 | 87.4% | 92.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 828
- ORF length:
- 762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agccaagacc ccaatttacc tgaaagcagc caataacaag aaaggaaaga 121 aatttaaact gagggacatt ctgtctcctg atatgatcag tcccccgctt ggagactttc 181 gccacaccat ccacattggc aaagagggcc agcacgatgt ctttggagat atttcctttc 241 ttcaagggaa ctacgagctt ttacctggaa accaggagaa agcacacctg ggccagttcc 301 ctgggcataa tgagttcttc cgggccaaca gcacctcgga ctctgtgttc acagaaacgc 361 cTCCCCCGGT GCTCAAAAAT GCCATCTCCC TCCCGACCAT TGGAGGATCC CAAGCTCTCA 421 TGTTGCCCTT ATTGTCACCA GTGACATTTA ATTCCAAACA GGAGTCCTTC GGGCCAGCAA 481 AGCTGCCCAG GCTTAGCTGC GAGCCCGTCA TGGAGGAAAA AGCTCAGGAG AAAAGCAGTC 541 TGTTGGAGAA TGGGACAGTC CACCAGGGAG ACACCTCGTG GGGCTCCAGC GGTTCTGCAT 601 CTCAGTCCAG CCAAGGCAGA GACAGCCACT CCTCCAGCCT GTCCGAACAG TACCCCGACT 661 GGCCAGCCGA GGACATGTTT GACCATCCCA CCCCATGCGA GCTCATCAAG GGAAAGACTA 721 AGTCAGAGGA GTCCCTCTCT GACCTTACAG GTTCCCTCCT CTCCCTGCAG CTTGATCTTG 781 GGCCCTCACT TTTGGATGAG GTGCTGAATG TAATGGATAA AAATAAGTAC CCAACTTTCT 841 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 901 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTGATT TCTTGGCTTT ATATTCTTGT 961 GGAAAGGACG AGTGTCGGGT TTGCTATCGA GTCCCACGCG TTAAGTCgac aatcaacctc 1021 tggattacaa aatttgtgaa agatt