Construct: ORF TRCN0000471904
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011878.1_s317c1
- Derived from:
- ccsbBroadEn_10560
- DNA Barcode:
- CCCAGTGTTTGCAGAGGTTGTTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ZNF295-AS1 (150142)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471904
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150142 | ZNF295-AS1 | ZNF295 antisense RNA 1 | NR_027273.2 | 52.7% | 1_167del;261T>C;579_777del | |
| 2 | human | 150142 | ZNF295-AS1 | ZNF295 antisense RNA 1 | NR_119385.1 | 49.8% | 1_213del;307T>C;625_823del | |
| 3 | human | 150142 | ZNF295-AS1 | ZNF295 antisense RNA 1 | NR_119384.1 | 40.7% | 1_396del;490T>C;808_1006del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 501
- ORF length:
- 411
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAAGATAA AATGTGGTGT GAGGACACGG 121 CCCAGCCCCA CCGTCGTCTT CCAGCCCCAC CATCGTCTTC CAGCCCCACC GTCGTCTTCC 181 AGCCCCACCG TCGTCTTCTA GCCCCACCAT CGTCGTCTTG GACTCTCTGG GTGGCACCAC 241 GCTGTGGGTG CTGCTCTCCT CTGTGTCCCA AACGAGTTTG TGCCTCACTT TGTCGGGTTT 301 TGGCTGTGAC TTGGAGCCTC ACCAAGCTCC CTTTTCCTCT GTCTCCCATT CTCCTCTCCA 361 GGCCAGGGTG GCTTCCCCAG CCCTCCTCCC CGGGTCCTGC CTGTGCCGAG CCTTCCTCCC 421 AGGAGGGAGG TGATACTGAC TTGAGATCTT ATGGGTGTTT TGTTTGTGGC TGGGCCTGGC 481 CGACCCTCTC TCCACGGCCT TAACCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCCA GTGTTTGCAG 661 AGGTTGTTTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt