Construct: ORF TRCN0000471906
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013871.1_s317c1
- Derived from:
- ccsbBroadEn_13286
- DNA Barcode:
- GCCCGGTTATATCTATATCTCTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGD5 (152273)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471906
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152273 | FGD5 | FYVE, RhoGEF and PH domain ... | NM_152536.4 | 36.9% | 36.9% | 1_2766del;2943G>T |
| 2 | human | 152273 | FGD5 | FYVE, RhoGEF and PH domain ... | XM_011533422.1 | 36.8% | 36.8% | 1_2766del;2943G>T;3936_3941delAGTTAC |
| 3 | human | 152273 | FGD5 | FYVE, RhoGEF and PH domain ... | NM_001320276.1 | 33.9% | 33.9% | 1_2766del;2943G>T;4065_4066ins129 |
| 4 | human | 152273 | FGD5 | FYVE, RhoGEF and PH domain ... | XM_011533423.1 | 33.9% | 33.9% | (many diffs) |
| 5 | mouse | 232237 | Fgd5 | FYVE, RhoGEF and PH domain ... | NM_172731.3 | 29.9% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1686
- ORF length:
- 1620
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggccttggat gacatggacc atgaaggcag agacacattg gcccgggagg 121 agctgaggca gggcctgagt gaactcccag ccatccacga ccttcatcaa ggcatcctgg 181 aggagctgga ggaaaggctg tcaaattggg agagccagca gaaggtagct gacgtcttcc 241 ttgcccggga gcaggggttt gatcaccacg ccactcacat cctgcagttc gacaggtacc 301 taggtctgct cagtgagaat tgcctccact ctccccggct ggcagctgct gtccgtgaat 361 ttgagcagag tgtacaagga ggcagccaga ctgcgaagca tcggctgctg cgggtggttc 421 aacgcctctt ccagtaccaa gtgctcctca cagactattt aaacaacctt tgtccggact 481 ccgccgagta cgacaacaca cagggtgcac tgagcctcat ctccaaagtc acagaccgtg 541 ccaacgacag catggagcaa ggggaaaacc tgcagaagct ggtccacatt gagcacagcg 601 tccggggcca aggggatctc ctccagccag gaagggagtt tctgaaggaa gggacgctga 661 tgaaagtaac agggaaaaac agacggcccc ggcacctatt tctgatgaac gatgtgctcc 721 tgtacaccta tccccagaag gatgggaagt accggctgaa gaacacattg gctgtggcca 781 acatgaaggt cagccgccct gtgatggaga aagtgcccta cgctctaaag attgagactt 841 ccgagtcctg cctgatgctg tctgcgagct cctgtgcaga gagggacgag tggtatggct 901 gtctgagcag agccctccct gaggactaca aggcccaggc gctggctgca ttccaccata 961 gcgtggagat acgagagagg ctgggggtta gccttgggga gaggcccccc accctggtgc 1021 ctgtcacaca cgtcatgatg tgcatgaact gcggctgcga cttctccctc accctgcggc 1081 gtcatcactg tcacgcctgt ggcaagatcg tgtgccggaa ctgttcgcgg aacaagtacc 1141 cgctgaagta cctgaaggac aggatggcca aggtctgcga cggctgcttc ggggagctga 1201 agaagcgggg cagggctgtc ccgggcctga tgagagagcg gccTGTGAGC ATGAGCTTCC 1261 CGCTGTCTTC ACCCCGCTTC TCGGGCAGTG CCTTTTCATC CGTCTTCCAG AGCATTAACC 1321 CCTCGACCTT CAAGAAGCAG AAGAAAGTCC CTTCAGCCCT GACAGAGGTG GCTGCCTCTG 1381 GAGAGGGCTC TGCCATCAGT GGCTATCTCA GCCGGTGTAA GAGGGGCAAG CGGCACTGGA 1441 AGAAGCTCTG GTTTGTCATC AAAGGCAAAG TTCTCTACAC CTACATGGCC AGTGAGGACA 1501 AAGTGGCCTT GGAGAGTATG CCTCTGCTAG GCTTCACCAT TGCTCCAGAA AAGGAAGAGG 1561 GCAGCAGTGA AGTAGGACCT ATTTTTCACC TTTACCACAA GAAAACCCTA TTTTATAGCT 1621 TCAAAGCAGA AGATACCAAT TCAGCTCAGA GGTGGATCGA GGCCATGGAA GATGCGAGTG 1681 TGTTATACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1741 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1801 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGCCCGGTTA TATCTATATC TCTATACGCG 1861 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt