Construct: ORF TRCN0000471907
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004183.1_s317c1
- Derived from:
- ccsbBroadEn_12647
- DNA Barcode:
- AGTACCTCGTGCCTCGACAAATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- L2HGDH (79944)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471907
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | NM_024884.3 | 91.6% | 83.8% | (many diffs) |
| 2 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_005268075.5 | 91.6% | 83.8% | (many diffs) |
| 3 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_011537166.3 | 83% | 74.4% | (many diffs) |
| 4 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_017021655.2 | 83% | 74.4% | (many diffs) |
| 5 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_011537167.3 | 82% | 74.2% | (many diffs) |
| 6 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_017021658.1 | 53.2% | 53% | (many diffs) |
| 7 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_011537168.3 | 52.7% | 46.2% | (many diffs) |
| 8 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_017021656.2 | 52.7% | 46.2% | (many diffs) |
| 9 | human | 79944 | L2HGDH | L-2-hydroxyglutarate dehydr... | XM_017021657.2 | 52.7% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1389
- ORF length:
- 1323
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gccagcgctg cgttatttgg ttggtgcctg cggacgggcc cgcgggcgtt 121 tcgccggtgg ctcccctggg gcgtgcgggt tcgcgtctgg gaggccaaga ccgctgtgtg 181 gaggtagccg cagcgccagc accagctcat ttgatatagt cattgttggt ggcggaattg 241 tggggcttgc ctctgccaga gcactcatcc tgcgacatcc atcactttct attggtgttc 301 tggaaaagga gaaagattta gctgttcacc agactggaca taacagtggt gtcatacata 361 gtggaattta ttataaacct gagtctctga aagccaaatt atgtgtacaa ggtgcagccc 421 tcctctatga gtactgtcag caaaagggaa tttcctacaa gcagtgtggc aagcttatag 481 tagctgttga acaagaagaa attcccagac ttcaggccct atatgagaaa ggcctccaga 541 atggtgtccc gggcctgagg ctgatccagc aggaggatat aaaaaagaag gagccatatt 601 gtaggggtct aatggctatt gattgtccac atactggcat tgtggactat cggcaggtgg 661 ctttgtcatt tgcccaggat ttccaagaag caggtggctc tgtcttgacc aattttgaag 721 taaaaggtat tgaaatggct aaagaaagtc cttcaagaag tatagatgga atgcaatatc 781 caattgttat aaagaataca aagggagagg aaattcgatg tcagtatgtt gtgacatgtg 841 caggacttta ctcagaccgt atttcagagt tgagtggctg cactcctgat cctcgaattg 901 taccattccg GGGAGATTAC CTGCTTTTGA AGCCAGAAAA ATGTTATCTT GTAAAAGGAA 961 ATATTTATCC GGTCCCAGAT AGCCGGTTTC CTTTCCTAGG AGTTCACTTC ACACCAAGGA 1021 TGGATGGCAG TATTTGGCTA GGGCCTAATG CAGTTCTTGC CTTTAAACGA GAGGGTTACA 1081 GACCCTTTGA CTTCAGTGCC ACAGATGTTA TGGATATAAT TATCAATAGT GGCTTGATTA 1141 AACTGGCATC CCAGAATTTT TCCTATGGAG TTACTGAAAT GTATAAAGCA TGTTTTCTTG 1201 GTGCAACAGT GAAGTATCTT CAAAAATTCA TCCCTGAAAT TACTATCAGT GATATACTTA 1261 GGCAGGTGGC TGTGAGAGGA CCGAGCTGGC TATGGCAGCA ACCCATGAAA GTGAGTGATA 1321 ACAACATTTA TTGTTTCCTG TGGCGTTGCT TTGCTTTGCT ATTGACTGGT AGCACTTGTA 1381 GCTTTAAATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1441 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1501 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTACC TCGTGCCTCG ACAAATCAAC 1561 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt