Construct: ORF TRCN0000471915
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010098.1_s317c1
- Derived from:
- ccsbBroadEn_13846
- DNA Barcode:
- TGGACTAGCCTACATAATATCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPH1 (1801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471915
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1801 | DPH1 | diphthamide biosynthesis 1 | NM_001346576.1 | 83.3% | 83.4% | 0_1ins180;591T>C |
| 2 | human | 1801 | DPH1 | diphthamide biosynthesis 1 | NM_001383.5 | 82.8% | 82.8% | 1_225del;996T>C |
| 3 | human | 1801 | DPH1 | diphthamide biosynthesis 1 | NM_001346574.1 | 77.1% | 77.2% | 1_240del;858_859ins63;948T>C |
| 4 | human | 1801 | DPH1 | diphthamide biosynthesis 1 | NM_001346575.1 | 74.6% | 74.7% | (many diffs) |
| 5 | human | 1801 | DPH1 | diphthamide biosynthesis 1 | NR_144474.1 | 42.2% | (many diffs) | |
| 6 | human | 1801 | DPH1 | diphthamide biosynthesis 1 | NR_144476.1 | 41.8% | (many diffs) | |
| 7 | human | 1801 | DPH1 | diphthamide biosynthesis 1 | NR_144475.1 | 40.9% | (many diffs) | |
| 8 | mouse | 116905 | Dph1 | diphthamide biosynthesis 1 | NM_144491.2 | 69.8% | 71.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1155
- ORF length:
- 1089
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggaaggcctc ctcctctttg cctgtaccat tgtggatatc ttggaaaggt 121 tcacggaggc cgaagtgatg gtgatgggtg acgtgaccta cggggcttgc tgtgtggatg 181 acttcacagc gagggccctg ggagctgact tcttggtgca ctacggccac agttgcctga 241 ttcccatgga cacctcggcc caagacttcc gggtgctgta cgtctttgtg gacatccgga 301 tagacactac acacctcctg gactctctcc gcctcacctt tcccccagcc actgcccttg 361 ccctggtcag caccattcag tttgtgtcga ccttgcaggc agccgcccag gagctgaaag 421 ccgagtatcg tgtgagtgtc ccacagtgca agcccctgtc ccctggagag atcctgggct 481 gcacatcccc ccgactgtcc aaagaggtgg aggccgttgt gtatcttgga gatggccgct 541 tccatctgga gtctgtcatg attgccaacc ccaatgtccc cgcttaccgg tatgacccat 601 atagcaaagt cctatccaga gaacactatg accaccagcg catgcaggct gctcgccaag 661 aagccatagc cactgcccgc tcagctaagt cctggggCCT TATTCTGGGC ACTTTGGGCC 721 GCCAGGGCAG TCCTAAGATC CTGGAGCACC TGGAATCTCG ACTCCGAGCC TTGGGCCTTT 781 CCTTTGTGAG GCTGCTGCTC TCTGAGATCT TCCCCAGCAA GCTTAGCCTA CTTCCCGAGG 841 TGGATGTGTG GGTGCAGGTG GCATGTCCAC GTCTCTCCAT TGACTGGGGC ACAGCCTTCC 901 CCAAGCCGCT GCTGACACCC TATGAGGCGG CCGTGGCTCT GAGGGACATT TCCTGGCAGC 961 AGCCCTACCC GATGGACTTC TACGCTGGCA GCTCCTTGGG GCCCTGGACG GTGAACCACG 1021 GCCAGGACCG CCGTCCCCAC GCCCCGGGCC GGCCCGCGCG GGGGAAGGTG CAGGAGGGGT 1081 CCGCGCGTCC CCCTTCGGCC GTGGCTTGCG AGGACTGCAG CTGCAGGGAC GAGAAGGTGG 1141 CGCCGCTGGC TCCTTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1201 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1261 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGGACTAGCC TACATAATAT 1321 CCTTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt