Construct: ORF TRCN0000471931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008437.1_s317c1
- Derived from:
- ccsbBroadEn_03603
- DNA Barcode:
- ACCCCTATGCAGCTGAGGTGGTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLK15 (55554)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471931
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55554 | KLK15 | kallikrein related peptidas... | NM_017509.3 | 100% | 100% | |
| 2 | human | 55554 | KLK15 | kallikrein related peptidas... | XM_006723265.3 | 100% | 100% | |
| 3 | human | 55554 | KLK15 | kallikrein related peptidas... | NM_001277081.1 | 99.6% | 99.6% | 40_41insCAG |
| 4 | human | 55554 | KLK15 | kallikrein related peptidas... | XM_011527085.2 | 99.6% | 99.6% | 40_41insCAG |
| 5 | human | 55554 | KLK15 | kallikrein related peptidas... | XM_011527087.2 | 66.7% | 66.7% | 361_362ins255 |
| 6 | human | 55554 | KLK15 | kallikrein related peptidas... | XM_011527088.2 | 62.7% | 62.5% | 482G>T;483_484ins285 |
| 7 | human | 55554 | KLK15 | kallikrein related peptidas... | NM_001277082.1 | 62.3% | 62.1% | 40_41insCAG;479G>T;480_481ins285 |
| 8 | human | 55554 | KLK15 | kallikrein related peptidas... | XM_011527089.2 | 62.3% | 62.1% | 40_41insCAG;479G>T;480_481ins285 |
| 9 | human | 55554 | KLK15 | kallikrein related peptidas... | NR_102274.1 | 48.8% | (many diffs) | |
| 10 | human | 55554 | KLK15 | kallikrein related peptidas... | XM_017026943.1 | 47.2% | 47.2% | 363_364ins405 |
| 11 | human | 55554 | KLK15 | kallikrein related peptidas... | XR_001753713.1 | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gcttctcctc actctctcct tcctgctggc atccacagca gcccaggatg 121 gtgacaagtt gctggaaggt gacgagtgtg caccccactc ccagccatgg caagtggctc 181 tctacgagcg tggacgcttt aactgtggcg cttccctcat ctccccacac tgggtgctgt 241 ctgcggccca ctgccaaagc cgcttcatga gagtgcgcct gggagagcac aacctgcgca 301 agcgcgatgg cccagagcaa ctacggacca cgtctcgggt cattccacac ccgcgctacg 361 aagcgcgcag ccaccgcaac gacatcatgt tgcTGCGCCT AGTCCAGCCC GCACGCCTGA 421 ACCCCCAGGT GCGCCCCGCG GTGCTACCCA CGCGTTGCCC CCACCCGGGG GAGGCCTGTG 481 TGGTGTCTGG CTGGGGCCTG GTGTCCCACA ACGAGCCTGG GACCGCTGGG AGCCCCCGGT 541 CACAAGTGAG TCTCCCAGAT ACGTTGCATT GTGCCAACAT CAGCATTATC TCGGACACAT 601 CTTGTGACAA GAGCTACCCA GGGCGCCTGA CAAACACCAT GGTGTGTGCA GGCGCGGAGG 661 GCAGAGGCGC AGAATCCTGT GAGGGTGACT CTGGGGGACC CCTGGTCTGT GGGGGCATCC 721 TGCAGGGCAT TGTGTCCTGG GGTGACGTCC CTTGTGACAA CACCACCAAG CCTGGTGTCT 781 ATACCAAAGT CTGCCACTAC TTGGAGTGGA TCAGGGAAAC CATGAAGAGG AACTGCCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAA CCCCTATGCA GCTGAGGTGG TTAACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att