Construct: ORF TRCN0000471942
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017253.1_s317c1
- Derived from:
- ccsbBroadEn_03045
- DNA Barcode:
- CATGTTCAGAAGTCGTTTACGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COA3 (28958)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471942
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28958 | COA3 | cytochrome c oxidase assemb... | NM_001040431.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 408
- ORF length:
- 318
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCGTCTTC GGGAGCTGGT GACCCTCTGG 121 ATTCTAAGCG TGGAGAGGCC CCGTTCGCTC AGCGTATCGA CCCGACTCGG GAGAAGCTGA 181 CACCCGAGCA ACTGCATTCC ATGCGGCAGG CGGAGCTTGC CCAGTGGCAG AAGGTCCTAC 241 CACGGCGGCG AACCCGGAAC ATCGTGACCG GCCTAGGCAT CGGGGCCCTG GTGTTGGCTA 301 TTTATGGTTA CACCTTCTAC TCGATTTCCC AGGAGCGTTT CCTAGATGAG CTAGAAGACG 361 AGGCCAAAGC TGCCCGAGCC CGAGCTCTGG CAAGGGCGTC AGGGTCCTAC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGACATGTTC AGAAGTCGTT TACGGATACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt