Construct: ORF TRCN0000471950
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011955.1_s317c1
- Derived from:
- ccsbBroadEn_09015
- DNA Barcode:
- GCCGATTGCTAGAACATAATCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TM2D3 (80213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471950
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_078474.3 | 99.8% | 99.5% | 17T>G |
| 2 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_025141.3 | 89.3% | 89% | 17T>G;90_91ins78 |
| 3 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_001308026.1 | 82.7% | 75.4% | (many diffs) |
| 4 | human | 80213 | TM2D3 | TM2 domain containing 3 | NM_001307960.1 | 72.2% | 65.3% | (many diffs) |
| 5 | mouse | 68634 | Tm2d3 | TM2 domain containing 3 | NM_178056.3 | 80% | 82.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 807
- ORF length:
- 741
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggaggggtg cgcccgctga ggggcctccg cgccttgtgt cgcgtgctgc 121 tcttcctctc gcagttctgc attctgtcgg gcggtgagca atcgcaggcg ctggctcagt 181 caataaagga tccgggccca acacgcacat tcacagtagt tcccagggca gcagaaagta 241 ctgaaatccc accttatgtg atgaagtgtc cgagcaatgg tttgtgtagc aggcttcctg 301 cagactgtat agactgcaca acaaatttct cctgtaccTA TGGGAAGCCT GTCACTTTTG 361 ACTGTGCAGT GAAACCATCT GTTACCTGTG TTGATCAAGA CTTCAAATCC CAAAAGAACT 421 TCATCATTAA CATGACTTGC AGATTTTGCT GGCAGCTTCC TGAAACAGAT TACGAGTGTA 481 CCAACTCCAC CAGCTGCATG ACGGTGTCCT GTCCTCGGCA GCGCTACCCT GCCAACTGCA 541 CGGTGCGGGA CCACGTCCAC TGCTTGGGTA ACCGTACTTT TCCCAAAATG CTATATTGCA 601 ATTGGACTGG AGGCTATAAG TGGTCTACGG CTCTGGCTCT AAGCATCACC CTCGGTGGGT 661 TTGGAGCAGA CCGTTTCTAC CTGGGCCAGT GGCGGGAAGG CCTCGGCAAG CTCTTCAGCT 721 TCGGTGGCCT GGGAATATGG ACGCTGATAG ACGTCCTGCT CATTGGAGTT GGCTATGTTG 781 GACCAGCAGA TGGCTCTTTG TACATTTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCGATTG 961 CTAGAACATA ATCTCCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1021 aagatt