Construct: ORF TRCN0000471965

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008995.1_s317c1
Derived from:
ccsbBroadEn_09637
DNA Barcode:
CTTAATAAGGCATCGCCATATAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
UNC45B (146862)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471965
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 146862 UNC45B unc-45 myosin chaperone B NM_001033576.2 99.9% 99.8% 178G>A
2 human 146862 UNC45B unc-45 myosin chaperone B NM_001267052.2 99.9% 99.8% 178G>A
3 human 146862 UNC45B unc-45 myosin chaperone B NM_173167.3 99.7% 99.6% 178G>A;1690_1695delGCAGGT
4 human 146862 UNC45B unc-45 myosin chaperone B NM_001308281.1 91.4% 91.3% 178G>A;1451_1452ins237
5 human 146862 UNC45B unc-45 myosin chaperone B XM_017024234.1 91.4% 91.3% 178G>A;1451_1452ins237
6 mouse 217012 Unc45b unc-45 myosin chaperone B NM_178680.4 88.7% 95% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2856
ORF length:
2787
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggcagaggtg gaagcggtac agctgaagga ggaaggaaac cggcatttcc
121agctccagga ctacaaggcc gccacaaata gctacagcca ggccctgaag ctgaccaagg
181acaaggccct gctggccacg ctttatcgga accgggcagc ctgtggcctg aaaacggaga
241gctacatcca ggcagcttca gatgcctcca gagccatcga catcaactcc tcggacatca
301aggctctgta tcggcgatgc caggcactgg agcacctggg gaagctggac caggccttca
361aagacgtgca gcgttgtgcc accctcgagc cacggaacca gaacttccag gagatgctga
421ggagactcaa caccagcatt caggagaagc tccgagtgca gttctccaca gactcgaggg
481tacagaagat gtttgagatc ctcttggatg aaaacagtga ggctgataag cgggaaaagg
541ctgccaacaa tctcattgtc ctaggccgtg aggaagcagg ggctgagaag atcttccaga
601acaatggagt agccttgcta ctgcagcttc tggacactaa gaagcctgag ctggtgctgg
661ctgcagtgcg gaccctgtcg ggcatgtgca gcggccacca agccagagcc acagtgattc
721tgcatgcagt gcggatagac cgaatctgta gcctcatggc cgtggagaat gaggagatgt
781ctctggctgt ctgcaacctg ctccaagcca tcattgactc cttgtctggg gaggacaagc
841gggagcatcg agggaaggag gaggccctgg ttctagacac caagaaggac ctgaagcaga
901tcaccagcca cctgctggac atgctagtca gcaagaaggt gtctggccag ggcagggatc
961aggcgctgaa cctgctcaat aagaatgttc ccaggaagga ccttgccatt catgacaact
1021cacgtaccat ctatgtggtg gataatggtc tgaggaagat cctgaaggtt gtggggcagg
1081ttccagatct gccatcctgc ctgcccctga ctgacaacac ccgcatgctg gcctctatcc
1141tcatcaacaa gctctatgat gacctgcgct gtgacccgga gcgcgatcac ttccgcaaga
1201tctgtgagga atatatcacg ggcaagtttg acccccagga catggacaag aacttgaatg
1261ccatccagac agtgtcaggg atcctgcagg gcccctttga cctgggcaac cagctgctgg
1321gactgaaagg tgtgatggag atgatggtgg cactatgtgg ctcagagcgc gagacggacc
1381agctggtggc cgtggaggcc ctcatccatg cctccacgaa gctcagccgc gccaccttca
1441tcatcaccaa tggagtgtca ctgctcaaac agatctacaa gaccaccaaa aatgagaaga
1501tcaagatccg cacactggtg ggactctgta agctcggctc tgcaggtggc acagactacg
1561gtctcaggca gtttgcggaa gggtcgacag aaaaactggc caaacagtgt cgcaagtggc
1621tgtgcaatat gtccatagac actcggaccc gacgctgggc agtggagggc ctggcctacc
1681tcacgctgga cgctgatgtg aaggacgact ttgtccagga cgtccctgcc ctgcaggcca
1741tgtttgagct ggccaagacc agtgacaaga ccatcctgta ctcggtggcc accaccctgg
1801tgaactgcac caacagctac gatgtcaagg aggtcatccc agagcttgtc cagctcgcca
1861agttctccaa gcagcatgtg cccgaggaac accccaagga caagaaggac tttatagaca
1921tgcgggtgaa gcggcttctg aaggcgggtg tcatctctgc cctggcttgc atggtgaaag
1981cagatagtgc catcctcact gaccagacca aggagctgct ggccagggta ttcctggcac
2041tgtgtgacaa cccaaaggac cgaggcacca ttgtggctca aggtggtggc aaggccctga
2101ttcccctggc tttggagggc acagatgtgg gcaaggtgaa ggcagcccac gctctagcaa
2161agatcgctgc tgtctccaat ccggacattg cttttcctgg ggagcgggtg tatgaggtgg
2221tgcggcccct tgtaagactc ttggacacac agagggatgg gcttcagaac tatgaggctc
2281tcctaggcct caccaacctg tctgggcgga gtgacaaact ccggcagaag atcTTTAAGG
2341AGAGGGCCTT GCCAGACATC GAGAACTACA TGTTTGAGAA TCATGATCAG CTGCGGCAGG
2401CGGCCACCGA GTGCATGTGC AACATGGTGC TCCACAAGGA GGTACAGGAA AGGTTCTTGG
2461CTGACGGGAA TGACCGGCTG AAGCTGGTGG TGCTGCTCTG CGGGGAGGAT GATGATAAGG
2521TGCAGAATGC GGCTGCAGGG GCTCTGGCCA TGCTGACAGC AGCACACAAG AAACTGTGCC
2581TCAAGATGAC TCAAGTGACA ACCCAGTGGT TGGAGATCCT CCAGCGGCTT TGCCTGCACG
2641ACCAGCTGTC TGTCCAACAC CGGGGCCTGG TCATTGCCTA CAACCTACTG GCAGCCGATG
2701CTGAGCTGGC CAAGAAGCTG GTGGAGAGTG AGCTGCTGGA GATCCTGACT GTGGTGGGCA
2761AACAGGAGCC AGATGAGAAG AAGGCAGAAG TGGTTCAGAC AGCCCGAGAA TGTCTCATCA
2821AGTGCATGGA TTATGGTTTC ATTAAACCAG TGTCTTTGCC AACTTTCTTG TACAAAGTGG
2881TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2941CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
3001ACTTAATAAG GCATCGCCAT ATAATACGCG TTAAGTCgac aatcaacctc tggattacaa
3061aatttgtgaa agatt

Download FASTA (ORF) (Full)