Construct: ORF TRCN0000471974
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018255.1_s317c1
- Derived from:
- ccsbBroadEn_13772
- DNA Barcode:
- CCAAGGACTACCATTCAAAATTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471974
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 101927905 | LINC02449 | long intergenic non-protein... | NR_120455.1 | 14.3% | (many diffs) | |
| 2 | human | 101927905 | LINC02449 | long intergenic non-protein... | NR_120454.1 | 12% | (many diffs) | |
| 3 | human | 285407 | ALG1L9P | ALG1 like 9, pseudogene | NR_073386.1 | 7% | (many diffs) | |
| 4 | human | 619423 | FAM85A | family with sequence simila... | NR_146925.1 | 6.2% | (many diffs) | |
| 5 | human | 112268389 | LOC112268389 | uncharacterized LOC112268389 | XR_002959144.1 | 3.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 314
- ORF end:
- 434
- ORF length:
- 120
- Sequence:
-
1 GTCGCTTCAT GTGACTCCAC GGAGTACCGG GCGCCGTCCA GGCACCTCGA TTAGTTCTCG 61 AGCTTTTGGA GTACGTCGTC TTTAGGTTGG GGGGAGGGGT TTTATGCGAT GGAGTTTCCC 121 CACACTGAGT GGGTGGAGAC TGAAGTTAGG CCAGCTTGGC ACTTGATGTA ATTCTCCTTG 181 GAATTTGCCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 241 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGCTAGCATC GATTGATCAA CAAGTTTGTA 301 CAAAAAAGTT GGCATGTCGT GGGAGATGAT GGCTGTGCTC TCGGAGAGTG TGACTGAGCC 361 TCCCGAGGGC TGCACCAAGA GAGGGCGAGA GAGTCAGTCC AGCAATCAGA AGGCAAGTGG 421 CTTAGAAGAC AAGTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC 481 TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT 541 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC CAAGGACTAC CATTCAAAAT 601 TCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag att