Construct: ORF TRCN0000471987

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013698.2_s317c1
Derived from:
ccsbBroadEn_05701
DNA Barcode:
CACAGATGCACTAACCCGGCGGCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CKMT1A (548596)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471987
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 548596 CKMT1A creatine kinase, mitochondr... NM_001015001.2 100% 100%
2 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321926.1 100% 100%
3 human 1159 CKMT1B creatine kinase, mitochondr... NM_020990.4 99.8% 100% 1056A>G;1086T>C
4 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521197.2 99.8% 100% 1056A>G;1086T>C
5 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321927.1 93% 93% 148_240del
6 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321928.1 93% 93% 148_240del
7 human 548596 CKMT1A creatine kinase, mitochondr... XM_017022369.1 93% 93% 148_240del
8 human 548596 CKMT1A creatine kinase, mitochondr... XM_017022370.1 93% 93% 148_240del
9 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521194.1 92.9% 93% 148_240del;1149A>G;1179T>C
10 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521195.2 92.9% 93% 148_240del;1149A>G;1179T>C
11 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521196.1 92.9% 93% 148_240del;1149A>G;1179T>C
12 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521198.1 88.7% 88.7% (many diffs)
13 human 548596 CKMT1A creatine kinase, mitochondr... NM_001321929.1 61.8% 60.9% 0_1ins433;11_12ins44
14 human 548596 CKMT1A creatine kinase, mitochondr... XM_005254498.4 61.8% 60.9% 0_1ins433;11_12ins44
15 human 1159 CKMT1B creatine kinase, mitochondr... XM_005254150.4 61.7% 60.9% (many diffs)
16 human 1159 CKMT1B creatine kinase, mitochondr... XM_011521199.2 61.7% 60.9% (many diffs)
17 human 1159 CKMT1B creatine kinase, mitochondr... XM_017021902.1 61.7% 60.9% (many diffs)
18 human 548596 CKMT1A creatine kinase, mitochondr... NR_135856.1 59.5% (many diffs)
19 human 1159 CKMT1B creatine kinase, mitochondr... NR_135751.1 46.2% (many diffs)
20 human 1159 CKMT1B creatine kinase, mitochondr... NR_135750.1 44.4% (many diffs)
21 human 1159 CKMT1B creatine kinase, mitochondr... NR_135748.1 43.7% (many diffs)
22 human 1159 CKMT1B creatine kinase, mitochondr... NR_135749.1 41.8% (many diffs)
23 human 548596 CKMT1A creatine kinase, mitochondr... XM_017022371.1 41.2% 34.5% 148_240del;537_538ins44;648_649ins652
24 mouse 12716 Ckmt1 creatine kinase, mitochondr... NM_001355069.1 92.2% 96.6% (many diffs)
25 mouse 12716 Ckmt1 creatine kinase, mitochondr... NM_009897.3 92.2% 96.6% (many diffs)
26 mouse 12716 Ckmt1 creatine kinase, mitochondr... XM_006498656.3 92.2% 96.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1317
ORF length:
1251
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tggtcccttc tcccgtctgc tgtccgcccg cccgggactc aggctcctgg
121ctttggccgg agcggggtct ctagccgctg ggtttctgct ccgaccggaa cctgtacgag
181ctgccagtga acgacggagg ctgtatcccc cgagcgctga gtacccagac ctccgaaagc
241acaacaactg catggccagt cacctgaccc cagcagtcta tgcacggctc tgcgacaaga
301ccacacccac tggttggacg ctagatcagt gtatccagac tggcgtggac aaccctggcc
361accccttcat caagactgtg ggcatggtgg ctggagatga ggagacctat gaggtatttg
421ctgacctgtt tgaccctgtg atccaagagc gacacaatgg atatgacccc cggacaatga
481agcacaccac ggatctagat gccagtaaaa tccgttctgg ctactttgat gagaggtatg
541tattgtcctc tagagtcaga actggccgaa gcatccgagg actcagtctg cctccagctt
601gcactcgagc agagcgacga gaggtggaac gtgttgtggt ggatgcactg agtggcctga
661agggtgacct ggctggacgt tactataggc tcagtgagat gacagaggct gaacagcagc
721agcttattga tgaccacttt ctgtttgata agcctgtgtc cccgttgctg actgcagcag
781gaatggctcg agactggcca gatgctcgtg gaatttggca caacaatgag aagagcttcc
841tgatctgggt gaatgaggag gatcatacac gggtgatctc catggagaag ggtggtaaca
901tgaagagagt gtttgaaaga ttctgccgag gccTCAAAGA GGTGGAGAGA CTTATCCAAG
961AACGTGGCTG GGAGTTCATG TGGAATGAGC GTTTGGGATA CATCTTGACC TGTCCATCTA
1021ACCTGGGCAC TGGACTTCGG GCAGGAGTGC ACATCAAACT GCCCCTGCTA AGCAAAGATA
1081GCCGCTTCCC AAAGATCCTG GAGAACCTAA GACTCCAAAA GCGTGGTACT GGAGGAGTGG
1141ACACTGCTGC CACAGGCGGT GTCTTTGATA TTTCTAATTT GGACCGACTA GGCAAATCAG
1201AGGTGGAGCT GGTGCAACTG GTCATCGATG GAGTAAACTA TTTGATTGAT TGTGAACGGC
1261GTCTGGAGAG AGGCCAGGAT ATCCGCATCC CCACACCTGT CATCCACACC AAGCATTACC
1321CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1381TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1441ATATATCTTG TGGAAAGGAC GACACAGATG CACTAACCCG GCGGCAACGC GTTAAGTCga
1501caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)