Construct: ORF TRCN0000471994
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001031.1_s317c1
- Derived from:
- ccsbBroadEn_12223
- DNA Barcode:
- AGCGGCCGATGCGGATAGGTGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDCA7L (55536)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471994
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55536 | CDCA7L | cell division cycle associa... | NM_018719.5 | 99.7% | 99.7% | 163_165delCAG |
| 2 | human | 55536 | CDCA7L | cell division cycle associa... | NM_001127370.3 | 92.2% | 92.2% | 0_1ins102;61_63delCAG |
| 3 | human | 55536 | CDCA7L | cell division cycle associa... | NM_001127371.3 | 90% | 90% | 164_165ins135 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1425
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gttggcgact cgctaccaga tccctaaaga agtggctgac atctttaacg 121 cccccagtga tgatgaagag tttgttggct tccgagatga tgttcccatg gaaaccctct 181 cgtcagagga gagctgcgat agttttgact cactagagtc agggaaacag gatgtgcgct 241 ttcattccaa atacttcaca gaagagctaa gaagaatttt tatagaggac actgactcag 301 agactgagga ttttgcagga tttacgcaga gtgatctgaa tggaaagact aacccagaag 361 taatggtcgt ggagtcagat ttgagtgatg atggcaaagc atctttggtg agcgaggaag 421 aggaagatga agaagaagat aaggctaccc ctagaagaag caggtctaga agaagtagta 481 ttggtcttcg agtagccttt cagttcccca ccaagaagct ggccaacaaa ccagataaaa 541 acagttcttc cgagcagttg ttttctagcg cacgcttaca gaatgagaaa aaaacaattc 601 ttgaaagaaa gaaagactgt agacaggtga tacaaaggga agattctacc tctgagtctg 661 aggatgactc tcgggatgag agccaggaga gttcagatgc tttgctgaaa aggaccatga 721 acatcaagga gaacaaagcc atgcttgccc agttattggc ggaattgaac tcgatgccag 781 atttcttccc agtacgaacc ccaacctcag cttctaggaa gaagacagtg aggcgggcct 841 tctcggaggg acagatcacg cggcgtatga acccaacccg gagtgcgcgg cctcctgaga 901 agtttgctct agagaacttc actgtctcag ccgctaaatt tgcggaagag ttttacagct 961 tccgaagaag gaagacaatt ggggggaaat gccgggagta cagacgacgt caccgtataT 1021 CTTCTTTTCG GCCAGTGGAG GATATCACCG AAGAGGACTT AGAAAATGTT GCCATAACTG 1081 TTCGAGATAA AATCTATGAT AAAGTTCTGG GTAACACGTG CCATCAGTGT CGACAAAAGA 1141 CCATCGACAC CAAGACAGTG TGTCGGAACC AGGGTTGCTG TGGTGTGCGA GGACAGTTCT 1201 GTGGACCATG CCTGCGGAAC CGCTATGGGG AGGATGTCAG ATCGGCATTG CTGGACCCGG 1261 ATTGGGTGTG TCCCCCCTGT CGTGGGATCT GCAATTGCAG CTACTGTCGG AAGCGTGACG 1321 GCCGCTGTGC CACAGGAATC CTCATTCATC TGGCCAAGTT TTATGGTTAT GACAATGTTA 1381 AGGAATATCT GGAGAGCTTA CAAAAGGAGC TGGTAGAAGA CAATTACCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1561 GAAAGGACGA AGCGGCCGAT GCGGATAGGT GTTCACGCGT TAAGTCgaca atcaacctct 1621 ggattacaaa atttgtgaaa gatt