Construct: ORF TRCN0000471996
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003183.1_s317c1
- Derived from:
- ccsbBroadEn_12720
- DNA Barcode:
- CCAGGTTCTCTACGCCAGAAACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CLPB (81570)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471996
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_001258393.3 | 47.2% | 46.7% | (many diffs) |
| 2 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_001258394.2 | 46.2% | 45.7% | (many diffs) |
| 3 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_001258392.3 | 45.2% | 44.7% | (many diffs) |
| 4 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | XM_005274320.1 | 45.1% | 44.6% | (many diffs) |
| 5 | human | 81570 | CLPB | ClpB homolog, mitochondrial... | NM_030813.6 | 43.3% | 42.8% | (many diffs) |
| 6 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_011241703.2 | 56.5% | 60.9% | (many diffs) |
| 7 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_006507489.3 | 55.9% | 60.3% | (many diffs) |
| 8 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_011241702.2 | 47.9% | 51.7% | (many diffs) |
| 9 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | NM_009191.3 | 40.3% | 43.5% | (many diffs) |
| 10 | mouse | 20480 | Clpb | ClpB caseinolytic peptidase B | XM_006507488.3 | 38.6% | 41.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1011
- ORF length:
- 945
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct aaaaagttgg tacttgcttg tttccacaca gggcttcatc aggctggaca 121 tgtccgagtt ccaggagcga cacgaggtgg ccaagtttat tgggtctcca ccaggctacg 181 ttggccatga ggagggtggc cagctgacca agaagttgaa gcagtgcccc aatgctgtgg 241 tgctctttga tgaagtagac aaggcccatc cagatgtgct caccatcatg ctgcagctgt 301 ttgatgaggg ccggctgaca gatggaaaag ggaagaccat tgattgcaag gacgccatct 361 tcatcatgac ctccaatgtg gccagcgacg agatcgcaca gcacgcgctg cagctgaggc 421 aggaagcttt ggagatgagc cgtaaccgta ttgccgaaaa cctgggggat gtccagataa 481 gtgacaagat caccatctca aagaacttca aggagaatgt gattcgccct atcctgaaag 541 ctcacttccg gagggatgag tttctgggac ggatcaatga gatcgtctac ttcctcccct 601 tctgccactc ggagctcatc caactcgtca acaaggaact aaacttctgg gCCAAGAGAG 661 CCAAGCAAAG GCACAACATC ACGCTGCTCT GGGACCGCGA GGTGGCAGAT GTGCTGGTCG 721 ACGGCTACAA TGTGCACTAT GGCGCCCGCT CCATCAAACA TGAGGTAGAA CGCCGTGTGG 781 TGAACCAGCT GGCAGCAGCC TATGAGCAGG ACCTGCTGCC AGGGGGCTGT ACTTTGCGCA 841 TCACGGTGGA GGACTCAGAC AAGCAGCTAC TCAAAAGCCC AGAACTGCCC TCACCCCAGG 901 CTGAGAAGCG CCTCCCCAAG CTGCGTCTGG AGATCATCGA CAAGGACAGC AAGACTCGCA 961 GACTGGACAT CCGGGCACCA CTGCACCCTG AGAAGGTGTG CAACACCATC TATCCAACTT 1021 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1081 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1141 CTTGTGGAAA GGACGACCAG GTTCTCTACG CCAGAAACAA ACGCGTTAAG TCgacaatca 1201 acctctggat tacaaaattt gtgaaagatt