Transcript: Human NM_030813.6

Homo sapiens ClpB homolog, mitochondrial AAA ATPase chaperonin (CLPB), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
CLPB (81570)
Length:
10053
CDS:
58..2181

Additional Resources:

NCBI RefSeq record:
NM_030813.6
NBCI Gene record:
CLPB (81570)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_030813.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000412557 CCTTTGTTGTGGGCCCATAAC pLKO_005 2375 3UTR 100% 10.800 15.120 N CLPB n/a
2 TRCN0000413329 TGATTCGCCCTATCCTGAAAG pLKO_005 1688 CDS 100% 10.800 15.120 N CLPB n/a
3 TRCN0000043565 CCGAGAGGATGACTTCAACAA pLKO.1 804 CDS 100% 4.950 6.930 N CLPB n/a
4 TRCN0000043564 GCGACTAAAGGAGCACATCAT pLKO.1 1080 CDS 100% 4.950 6.930 N CLPB n/a
5 TRCN0000043567 GCTCATCCAACTCGTCAACAA pLKO.1 1782 CDS 100% 4.950 6.930 N CLPB n/a
6 TRCN0000421374 GACACGAGGTGGCCAAGTTTA pLKO_005 1307 CDS 100% 13.200 9.240 N CLPB n/a
7 TRCN0000422261 TGCCCTCACCATCCAATAAAG pLKO_005 2204 3UTR 100% 13.200 9.240 N CLPB n/a
8 TRCN0000421594 ACGGCTACAATGTGCACTATG pLKO_005 1889 CDS 100% 10.800 7.560 N CLPB n/a
9 TRCN0000419827 AGTTTCTGGGACGGATCAATG pLKO_005 1727 CDS 100% 10.800 7.560 N CLPB n/a
10 TRCN0000417752 CAGAAGGTGCAGATGTCAATG pLKO_005 521 CDS 100% 10.800 7.560 N CLPB n/a
11 TRCN0000417540 GACAAGATCACCATCTCAAAG pLKO_005 1651 CDS 100% 10.800 7.560 N CLPB n/a
12 TRCN0000043563 GCCAAATATATGCACAAAGAT pLKO.1 1240 CDS 100% 5.625 3.938 N CLPB n/a
13 TRCN0000436601 AGTTCTGCAATTTAGTCTGTA pLKO_005 2676 3UTR 100% 4.950 3.465 N CLPB n/a
14 TRCN0000043566 GCTCTTTGATGAAGTAGACAA pLKO.1 1410 CDS 100% 4.950 3.465 N CLPB n/a
15 TRCN0000434205 GAACAGGGAATCCATTCTTTG pLKO_005 679 CDS 100% 10.800 6.480 N CLPB n/a
16 TRCN0000165534 GAGACAGGGTTTCACCATGTT pLKO.1 3673 3UTR 100% 4.950 2.475 Y n/a
17 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 3744 3UTR 100% 5.625 2.813 Y KLHL30 n/a
18 TRCN0000179120 CAACATGGTGAAACCCTGTTT pLKO.1 4035 3UTR 100% 4.950 2.475 Y LOC339059 n/a
19 TRCN0000166364 CACACACACACACACACACAA pLKO.1 8717 3UTR 100% 4.950 2.475 Y KAAG1 n/a
20 TRCN0000172742 GAGACAGAGTCTTGCTCTGTT pLKO.1 7060 3UTR 100% 0.495 0.248 Y C11orf44 n/a
21 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 3744 3UTR 100% 5.625 2.813 Y EID2B n/a
22 TRCN0000138772 GCAGGAGAATCGCTTGAACTT pLKO.1 6544 3UTR 100% 4.950 2.475 Y DCAF11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_030813.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_04240 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_04240 pLX_304 0% 100% 100% V5 n/a
3 ccsbBroadEn_12720 pDONR223 100% 43.3% 42.8% None (many diffs) n/a
4 ccsbBroad304_12720 pLX_304 0% 43.3% 42.8% V5 (many diffs) n/a
5 TRCN0000471996 CCAGGTTCTCTACGCCAGAAACAA pLX_317 38.3% 43.3% 42.8% V5 (many diffs) n/a
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