Construct: ORF TRCN0000472007
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008541.1_s317c1
- Derived from:
- ccsbBroadEn_08863
- DNA Barcode:
- TGGAATTCAGGCCGCCCGCCTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C16orf58 (64755)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472007
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64755 | C16orf58 | chromosome 16 open reading ... | NM_022744.4 | 99.9% | 99.7% | 554A>G |
| 2 | human | 64755 | C16orf58 | chromosome 16 open reading ... | XM_017023570.1 | 52.3% | 52.3% | 0_1ins669 |
| 3 | human | 64755 | C16orf58 | chromosome 16 open reading ... | XR_950831.3 | 48.3% | (many diffs) | |
| 4 | mouse | 233913 | BC017158 | cDNA sequence BC017158 | XM_011241783.1 | 59.8% | 58.1% | (many diffs) |
| 5 | mouse | 233913 | BC017158 | cDNA sequence BC017158 | XM_011241784.2 | 53.3% | 51.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgacgacgcg ggtttggaga ccccgctgtg ttccgagcag ttcggctccg 121 gggaggcacg gggctgccgc gccgccgcgg acgggagcct gcagtgggag gtcgggggct 181 ggcgctggtg ggggctctcc agggccttca cggtcaaacc tgaaggacga gatgcgggcg 241 aagtgggggc ttccggggcc ccctcaccgc ccctctccgg gctccaggcc gtgttcctgc 301 ctcagggctt ccctgatagc gtcagcccgg actacttgcc ctaccagctg tgggattccg 361 tgcaggcgtt tgcttccagc ctctccggct ccctagccac ccaggcagtc ttgctgggca 421 taggggtggg gaacgcaaaa gccactgttt cagctgccac ggccacctgg ctcgtgaaag 481 attcaactgg catgctgggc cgcatcgtct ttgcctggtg gaaagggagc aaactggact 541 gcaatgccaa gcagtggagg ctttttgcgg acatcctcaa tgacgtagcc atgttccttg 601 agattatggc tcctgtatgc ccaatctgtt tcaccatgac cgtctccacc agcaacctag 661 ccaagtgcat cgtgagtgtt gctggtgggg ccactcgggc tgccctgacc gtgcaccagg 721 ctcggaggaa caacatggct gacgtgtcag ccaaggacag cagccaggag acgctggtga 781 acctggcggg gctcttggtc agcctcctga tgctccctct ggtgtcaggt tgccctggct 841 tcagccttgg atgtttcttc ttcctcactg ccctccacat ctacgccaac taccgcgcgg 901 tccgagccct ggtcatggag accttgaacg aaggccggct ccggctggtc ctgaagcact 961 accttcagag gggagaggta ctcgacccaa ctgcagccaa tcgcatggag ccgctgtgga 1021 caggtttctg gccagcTCCG TCTCTATCCC TGGGGGTCCC CTTACACCGC TTGGTCTCCA 1081 GTGTCTTTGA GCTGCAGCAG CTGGTTGAGG GGCACCAAGA ATCCTACCTC CTCTGCTGGG 1141 ACCAGTCACA AAACCAGGTA CAGGTAGTTC TGAACCAGAA GGCAGGCCCC AAGACCATCC 1201 TAAGGGCCGC CACACATGGG CTGATGCTTG GGGCCCTGCA GGGAGATGGA CCCCTTCCAG 1261 CAGAGCTGGA GGAGCTGAGG AACCGGGTGC GGGCAGGTCC TAAGAAAGAG AGCTGGGTCG 1321 TCGTCAAGGA GACACACGAA GTGTTGGACA TGCTGTTCCC AAAGTTCTTG AAAGGACTGC 1381 AGGATGCCGG CTGGAAGACC GAGAAGCACC AGCTAGAGGT GGATGAGTGG AGGGCCACAT 1441 GGCTTCTGTC TCCCGAAAAG AAGGTCTTGT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1501 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1561 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGGAA 1621 TTCAGGCCGC CCGCCTTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1681 tgaaagatt