Construct: ORF TRCN0000472023
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014904.1_s317c1
- Derived from:
- ccsbBroadEn_01591
- DNA Barcode:
- GACCAATTTACCATTCTGCATGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR1D1 (6718)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472023
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6718 | AKR1D1 | aldo-keto reductase family ... | NM_005989.4 | 100% | 100% | |
| 2 | human | 6718 | AKR1D1 | aldo-keto reductase family ... | NM_001190907.2 | 88.9% | 87.7% | 855_856ins83;870_871ins25 |
| 3 | human | 6718 | AKR1D1 | aldo-keto reductase family ... | NM_001190906.2 | 87.4% | 87.4% | 454_455ins123 |
| 4 | mouse | 208665 | Akr1d1 | aldo-keto reductase family ... | XM_006505827.2 | 55.6% | 54.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1047
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatctcagt gctgcaagtc accgcatacc tctaagtgat ggaaacagca 121 ttcccatcat cggacttggt acctactcag aacctaaatc gacccctaag ggagcctgtg 181 caacatcggt gaaggttgct attgacacag ggtaccgaca tattgatggg gcctacatct 241 accaaaatga acacgaagtt ggggaggcca tcagggagaa gatagcagaa ggaaaggtgc 301 ggagggaaga tatcttctac tgtggaaagc tatgggctac aaatcatgtc ccagagatgg 361 tccgcccaac cctggagagg acactcaggg tcctccagct agattatgtg gatctttaca 421 tcattgaagt acccatggcc tttaagccag gagatgaaat ataccctaga gatgagaatg 481 gcaaatggtt atatcacaag tcaaatctgt gtgccacttg ggaggcgatg gaagcttgca 541 aagacgctgg cttggtgaaa tccctgggag tgtccaattt taaccgcagg cagctggagc 601 TCATCCTGAA CAAGCCAGGA CTCAAACACA AGCCAGTCAG CAACCAGGTT GAGTGCCATC 661 CGTATTTCAC CCAGCCAAAA CTCTTGAAAT TTTGCCAACA ACATGACATT GTCATTACTG 721 CATATAGCCC TTTGGGGACC AGTAGGAATC CAATCTGGGT GAATGTTTCT TCTCCACCTT 781 TGTTAAAGGA TGCACTTCTA AACTCATTGG GGAAAAGGTA CAATAAGACA GCAGCTCAAA 841 TTGTTTTGCG TTTCAACATC CAGCGAGGGG TGGTTGTCAT TCCTAAAAGC TTTAATCTTG 901 AAAGGATCAA AGAAAATTTT CAGATCTTTG ACTTTTCTCT CACTGAAGAA GAAATGAAGG 961 ACATTGAAGC CTTGAATAAA AATGTCCGCT TTGTAGAATT GCTCATGTGG CGCGATCATC 1021 CTGAATACCC ATTTCATGAT GAATACTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGACCAATT 1201 TACCATTCTG CATGGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt