Construct: ORF TRCN0000472036
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012419.1_s317c1
- Derived from:
- ccsbBroadEn_06806
- DNA Barcode:
- TCTCGCTGACATTAACTACATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMD3 (5709)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472036
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5709 | PSMD3 | proteasome 26S subunit, non... | NM_002809.4 | 99.8% | 99.8% | 141G>A;911A>T |
| 2 | mouse | 22123 | Psmd3 | proteasome (prosome, macrop... | NM_009439.1 | 89.3% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1668
- ORF length:
- 1602
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gcaggagggc tcggcgcggc gccgcggcgc ggacaaggcg aaaccgccgc 121 ccggcggagg agaacaagaa cccccaccgc cgccggcccc ccaggatgtg gagatgaaag 181 aggaggcagc gacgggtggc gggtcaacgg gggaggcaga cggcaagacg gcggcggcag 241 cggctgagca ctcccagcga gagctggaca cagtcacctt ggaggacatc aaggagcacg 301 tgaaacagct agagaaagcg gtttcaggca aggagccgag attcgtgctg cgggccctgc 361 ggatgctgcc ttccacatca cgccgcctca accactatgt tctgtataag gctgtgcagg 421 gcttcttcac ttcaaataat gccactcgag actttttgct ccccttcctg gaagagccca 481 tggacacaga ggctgattta cagttccgtc cccgcacggg aaaagctgcg tcgacacccc 541 tcctgcctga agtggaagcc tatctccaac tcctcgtggt catcttcatg atgaacagca 601 agcgctacaa agaggcacag aagatctctg atgatctgat gcagaagatc agtactcaga 661 accgccgggc cctagacctt gtagccgcaa agtgttacta ttatcacgcc cgggtctatg 721 agttcctgga caagctggat gtggtgcgca gcttcttgca tgctcggctc cggacagcta 781 cgcttcggca tgacgcagac gggcaggcca ccctgttgaa cctcctgctg cggaattacc 841 tacactacag cttgtacgac caggctgaga agctggtgtc caagtctgtg ttcccagagc 901 aggccaacaa caatgagtgg gccaggtacc tctactacac agggcgaatc aaagccatcc 961 agctggagta ctcagtggcc cggagaacga tgaccaacgc ccttcgcaag gcccctcagc 1021 acacagctgt cggcttcaaa cagacggtgc acaagcttct catcgtggtg gagctgttgc 1081 tgggggagat ccctgaccgg ctgcagttcc gccagccctc cctcaagcgc tcactcatgc 1141 cctatttcct tctgactcaa gctgtcagga caggaaacct agccaagttc aaccaggTCC 1201 TGGATCAGTT TGGGGAGAAG TTTCAAGCAG ATGGGACCTA CACCCTAATT ATCCGGCTGC 1261 GGCACAACGT GATTAAGACA GGTGTACGCA TGATCAGCCT CTCCTATTCC CGAATCTCCT 1321 TGGCTGACAT CGCCCAGAAG CTGCAGTTGG ATAGCCCCGA AGATGCAGAG TTCATTGTTG 1381 CCAAGGCCAT CCGGGATGGT GTCATTGAGG CCAGCATCAA CCACGAGAAG GGCTATGTCC 1441 AATCCAAGGA GATGATTGAC ATCTATTCCA CCCGAGAGCC CCAGCTAGCC TTCCACCAGC 1501 GCATCTCCTT CTGCCTAGAT ATCCACAACA TGTCTGTCAA GGCCATGAGG TTTCCTCCCA 1561 AATCGTACAA CAAGGACTTG GAGTCTGCAG AGGAACGGCG TGAGCGAGAA CAGCAGGACT 1621 TGGAGTTTGC CAAGGAGATG GCAGAAGATG ATGATGACAG CTTCCCTTGC CCCACTTTCT 1681 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1741 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1801 GTGGAAAGGA CGATCTCGCT GACATTAACT ACATGCAACG CGTTAAGTCg acaatcaacc 1861 tctggattac aaaatttgtg aaagatt