Construct: ORF TRCN0000472057
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018335.1_s317c1
- Derived from:
- ccsbBroadEn_03981
- DNA Barcode:
- ACCTGTGAGTGTGGATATTGGGTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CDCP1 (64866)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472057
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 64866 | CDCP1 | CUB domain containing prote... | NM_178181.2 | 100% | 100% | |
2 | human | 64866 | CDCP1 | CUB domain containing prote... | XM_017007070.1 | 43.5% | 43.3% | 1025A>G;1027A>G;1029_2358delinsG |
3 | human | 64866 | CDCP1 | CUB domain containing prote... | NM_022842.5 | 40.9% | 40.7% | 1025A>G;1027A>G;1029_2508delinsG |
4 | human | 64866 | CDCP1 | CUB domain containing prote... | XM_011534024.1 | 40.7% | 40.6% | (many diffs) |
5 | human | 64866 | CDCP1 | CUB domain containing prote... | XM_024453718.1 | 33% | 32.8% | (many diffs) |
6 | mouse | 109332 | Cdcp1 | CUB domain containing prote... | XM_006511922.3 | 29.4% | 30.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1095
- ORF length:
- 1029
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cggcctgaac tgcggggtct ctatcgcact gctaggggtt ctgctgctgg 121 gtgcggcgcg cctgccgcgc ggggcagaag cttttgagat tgctctgcca cgagaaagca 181 acattacagt tctcataaag ctggggaccc cgactctgct ggcaaaaccc tgttacatcg 241 tcatttctaa aagacatata accatgttgt ccatcaagtc tggagaaaga atagtcttta 301 cctttagctg ccagagtcct gagaatcact ttgtcataga gatccagaaa aatattgact 361 gtatgtcagg cccatgtcct tttggggagg ttcagcttca gccctcgaca tcgttgttgc 421 ctaccctcaa cagaactttc atctgggatg tcaaagctca taagagcatc ggtttagagc 481 tgcagttttc catccctcgc ctgaggcaga tcggtccggg tgagagctgc ccagacggag 541 tcactcactc catcagcggc cgaatcgatg ccaccgtggt caggatcgga accttctgca 601 gcaatggcac tgtgtcccgg atcaagatgc aagaaggagt gaaaatggcc ttacacctcc 661 catggttcca ccccagaaat gtctccggct tcagcattgc aaaccgctca tctataaaac 721 gtctgtgcat catcgagtct gtgtttgagg gtgaaggctc agcaaccctg atgtctgcca 781 actacccaga aggcttccct gaggatgagc tcatgacgtg gcagtttgtc gttcctgcac 841 acctgcgggc cagcgtctcc ttcctcaact tcaacctctc caactgtgag aggaaggagg 901 agcgggttga atactacatc ccgggctcca ccaccaaccc cgaggtgttc aagctggagg 961 acaagcagcc tgggaacatg gcggggaact tcaaCCTCTC TCTGCAAGGC TGTGACCAAG 1021 ATGCCCAAAG TCCAGGGATC CTCCGGCTGC AGTTCCAAGT TTTGGTCCAA CATCCACAAA 1081 ATGAAAGCAG TGAGTGAGCC CCACTTTCCT TTTTCTTCCT CCTCCAGCAC CTTCGTTGTT 1141 TCCTGGGTAG TCTGCCTGGG TGAGGCTCCC TTCCTGTTTC TCATCTGTGG CTTCTGAAAC 1201 ACTTAGACTC TGGACCCAGC AAGAGTTTCA GGAAGTGGGT TGCTAGGCAG TTAGACAGGC 1261 TTGTTGGTGA ACACCCGGTA TGTAGTTCCA TTTCAGCACA ATAAAAAGAA ATCTTGCATT 1321 CAAAAAAAAA AAAAAAAAAA CTCGAGGCAT CTATGTCGGG TGCGGAGAAA GAGGTAATGA 1381 AATGGCACAT GGTCATAGCT GTTTCCTGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC 1441 GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG 1501 TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACCTGTG 1561 AGTGTGGATA TTGGGTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg 1621 aaagatt