Construct: ORF TRCN0000472069
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015875.1_s317c1
- Derived from:
- ccsbBroadEn_12083
- DNA Barcode:
- TGGTGTGATTGTGCTCGTGCATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TET2 (54790)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472069
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54790 | TET2 | tet methylcytosine dioxygen... | XM_006714242.3 | 32.3% | 32.2% | 1_3861del;4984A>G |
| 2 | human | 54790 | TET2 | tet methylcytosine dioxygen... | NM_001127208.2 | 30.7% | 30.6% | 1_4161del;5284A>G |
| 3 | human | 54790 | TET2 | tet methylcytosine dioxygen... | XM_005263082.3 | 30.7% | 30.6% | 1_4161del;5284A>G |
| 4 | human | 54790 | TET2 | tet methylcytosine dioxygen... | XM_024454102.1 | 30.7% | 30.6% | 1_4161del;5284A>G |
| 5 | human | 54790 | TET2 | tet methylcytosine dioxygen... | XM_024454103.1 | 30.7% | 30.6% | 1_4161del;5284A>G |
| 6 | human | 54790 | TET2 | tet methylcytosine dioxygen... | XR_244633.3 | 19.9% | 1_4098del;5221A>G;5944_9230del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1914
- ORF length:
- 1845
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcagaatggc agcacattgg tatgcactct cactagagaa gacaatcgag 121 aatttggagg aaaacctgag gatgagcagc ttcacgttct gcctttatac aaagtctctg 181 acgtggatga gtttgggagt gtggaagctc aggaggagaa aaaacggagt ggtgccattc 241 aggtactgag ttcttttcgg cgaaaagtca ggatgttagc agagccagtc aagacttgcc 301 gacaaaggaa actagaagcc aagaaagctg cagctgaaaa gctttcctcc ctggagaaca 361 gctcaaataa aaatgaaaag gaaaagtcag ccccatcacg tacaaaacaa actgaaaacg 421 caagccaggc taaacagttg gcagaacttt tgcgactttc aggaccagtc atgcagcagt 481 cccagcagcc ccagcctcta cagaagcagc caccacagcc ccagcagcag cagagacccc 541 agcagcagca gccacatcac cctcagacag agtctgtcaa ctcttattct gcttctggat 601 ccaccaatcc atacatgaga cggcccaatc cagttagtcc ttatccaaac tcttcacaca 661 cttcagatat ctatggaagc accagcccta tgaacttcta ttccacctca tctcaagctg 721 caggttcata tttgaattct tctaatccca tgaaccctta ccctgggctt ttgaatcaga 781 atacccaata tccatcatat caatgcaatg gaaacctatc agtggacaac tgctccccat 841 atctgggttc ctattctccc cagtctcagc cgatggatct gtataggtat ccaagccaag 901 accctctgtc taagctcagt ctaccaccca tccatacact ttaccagcca aggtttggaa 961 atagccagag ttttacatct aaatacttag gttatggaaa ccaaaatatg cagggagatg 1021 gtttcagcag ttgtaccatt agaccaaatg tacatcatgt agggaaattg cctccttatc 1081 ccactcatga gatggatggc cacttcatgg gagccacctc tagattacca cccaatctga 1141 gcaatccaaa catggactat aaaaatggtg aacatcattc accttctcac gtaatccata 1201 actacagtgc agctccgggc atgttcaaca gctctcttca tgccctgcat ctccaaaaca 1261 aggagaatga catgctttcc cacacagcta atgggttatc aaagatgctt ccagctctta 1321 accatgatag aactgcttgt gtccaaggag gcttacacaa attaagtgat gctaatggtc 1381 aggaaaagca gccattggca ctagtccagg gtgtggcttc tggtgcagag gacaacgatg 1441 aggtctggtc agacagcgag cagagctttc tggatcctga cattggggga gtggccgtgg 1501 ctccaactca tgggtcaatt ctcattgagt gtgcaaagcg tgagctgcat gccacaaccc 1561 ctttaaagaa tcccaatagg aatcacccca ccaggatctc cctcgtcttt taccagcata 1621 agagcatgaa tgagccaaaa catggcttgg ctctttggga agccaaaatg gctgaaaaag 1681 cccgtgagaa agaggaagag tgtgaaaagt atggcccaga ctatgtgcct cagaaatccc 1741 atggcaaaaa agtgaaacgg gagcctgcTG AGCCACATGA AACTTCAGAG CCCACTTACC 1801 TGCGTTTCAT CAAGTCTCTT GCCGAAAGGA CCATGTCCGT GACCACAGAC TCCACAGTAA 1861 CTACATCTCC ATATGCCTTC ACTCGGGTCA CAGGGCCTTA CAACAGATAT ATATTGCCAA 1921 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1981 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2041 TATCTTGTGG AAAGGACGAT GGTGTGATTG TGCTCGTGCA TTGACGCGTT AAGTCgacaa 2101 tcaacctctg gattacaaaa tttgtgaaag att