Construct: ORF TRCN0000472075
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010865.1_s317c1
- Derived from:
- ccsbBroadEn_06430
- DNA Barcode:
- GGACGAGAACCTCTTCCGAGTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IFNA13 (3447)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472075
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3439 | IFNA1 | interferon alpha 1 | NM_024013.3 | 99.6% | 99.4% | 29T>C;94C>T |
| 2 | human | 3447 | IFNA13 | interferon alpha 13 | NM_006900.4 | 99.2% | 99.4% | 1_3delATG;97C>T |
| 3 | human | 3443 | IFNA6 | interferon alpha 6 | NM_021002.2 | 90.8% | 85.1% | (many diffs) |
| 4 | human | 3448 | IFNA14 | interferon alpha 14 | NM_002172.3 | 90.2% | 82.5% | (many diffs) |
| 5 | human | 3442 | IFNA5 | interferon alpha 5 | NM_002169.2 | 90.1% | 84.6% | (many diffs) |
| 6 | human | 3440 | IFNA2 | interferon alpha 2 | NM_000605.4 | 89.5% | 82.5% | (many diffs) |
| 7 | human | 3452 | IFNA21 | interferon alpha 21 | NM_002175.2 | 89.4% | 81.4% | (many diffs) |
| 8 | human | 3441 | IFNA4 | interferon alpha 4 | NM_021068.2 | 88.3% | 80.4% | (many diffs) |
| 9 | human | 3451 | IFNA17 | interferon alpha 17 | NM_021268.2 | 88.3% | 79.3% | (many diffs) |
| 10 | human | 3446 | IFNA10 | interferon alpha 10 | NM_002171.2 | 87.6% | 79.3% | (many diffs) |
| 11 | human | 3445 | IFNA8 | interferon alpha 8 | NM_002170.4 | 87.6% | 77.7% | (many diffs) |
| 12 | human | 3449 | IFNA16 | interferon alpha 16 | NM_002173.3 | 87.1% | 77.2% | (many diffs) |
| 13 | human | 3444 | IFNA7 | interferon alpha 7 | NM_021057.2 | 86.5% | 76.1% | (many diffs) |
| 14 | human | 3453 | IFNA22P | interferon alpha 22, pseudo... | NR_036676.1 | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcgcccttt gctttactga tggccctggt ggtgctcagc tgcaagtcaa 121 gctgctctct gggctgtgat ctccctgaga cccacagctt ggataacagg aggaccttga 181 tgctcctggc acaaatgagc agaatctctc cttcctcctg tctgatGGAC AGACATGACT 241 TTGGATTTCC CCAGGAGGAG TTTGATGGCA ACCAGTTCCA GAAGGCTCCA GCCATCTCTG 301 TCCTCCATGA GCTGATCCAG CAGATCTTCA ACCTCTTTAC CACAAAAGAT TCATCTGCTG 361 CTTGGGATGA GGACCTCCTA GACAAATTCT GCACCGAACT CTACCAGCAG CTGAATGACT 421 TGGAAGCCTG TGTGATGCAG GAGGAGAGGG TGGGAGAAAC TCCCCTGATG AATGCGGACT 481 CCATCTTGGC TGTGAAGAAA TACTTCCGAA GAATCACTCT CTATCTGACA GAGAAGAAAT 541 ACAGCCCTTG TGCCTGGGAG GTTGTCAGAG CAGAAATCAT GAGATCCCTC TCTTTATCAA 601 CAAACTTGCA AGAAAGATTA AGGAGGAAGG AATACCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGG 781 ACGAGAACCT CTTCCGAGTA AGACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt