Construct: ORF TRCN0000472076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013979.1_s317c1
- Derived from:
- ccsbBroadEn_10674
- DNA Barcode:
- CATCTATTTCCCGTCATATGACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKT2 (208)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472076
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526622.2 | 34.2% | 24.3% | (many diffs) |
| 2 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | NM_001330511.1 | 31.3% | 22.3% | (many diffs) |
| 3 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_024451417.1 | 31.3% | 22.3% | (many diffs) |
| 4 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | NM_001626.6 | 28.5% | 20.4% | (many diffs) |
| 5 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526614.1 | 28.5% | 20.4% | (many diffs) |
| 6 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526615.1 | 28.5% | 20.4% | (many diffs) |
| 7 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526616.1 | 28.5% | 20.4% | (many diffs) |
| 8 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526618.1 | 28.5% | 20.4% | (many diffs) |
| 9 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526619.1 | 28.5% | 20.4% | (many diffs) |
| 10 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_011526620.1 | 28.5% | 20.4% | (many diffs) |
| 11 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_017026470.2 | 28.5% | 20.4% | (many diffs) |
| 12 | human | 208 | AKT2 | AKT serine/threonine kinase 2 | XM_024451416.1 | 28.5% | 20.4% | (many diffs) |
| 13 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_001110208.2 | 26.9% | 20% | (many diffs) |
| 14 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_001331108.1 | 26.9% | 20% | (many diffs) |
| 15 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_001331109.1 | 26.9% | 20% | (many diffs) |
| 16 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | NM_007434.4 | 26.9% | 20% | (many diffs) |
| 17 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539477.1 | 26.9% | 20% | (many diffs) |
| 18 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539480.1 | 26.9% | 20% | (many diffs) |
| 19 | mouse | 11652 | Akt2 | thymoma viral proto-oncogene 2 | XM_006539481.3 | 26.9% | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gtgtctgtca tcaaagaagg ctggctccac aagcgtggtg aaatacatca 121 agacctggag gccacggtac ttcctgctga agagcgacgg ctccttcatt GGGTACAAGG 181 AGAGGCCCGA GGCCCCTGAT CAGACTCTAC CCCCCTTAAA CAACTTCTCC GTAGCAGAAT 241 GCCAGCTGAT GAAGACCGAG AGGCCGCGAC CCAACACCTT TGTCATACGC TGCCTGCAGT 301 GGACCACAGT CATCGAGAGG ACCTTCCACG TGGATTCTCC AGACGAGAGG GAGGAGTGGA 361 TGCGGGCCAT CCAGATGGTC GCCAACAGCC TCCAGCCTCA CCTTTGTGCC CAGACTCGCA 421 TTTGGAAGAC TCCACCTCCC GCCCAGGCCT GGGCTGTTGG GCGGTTGGAG ATTCAGGTTT 481 TAATCCACAC AAGCCCCAGT GAGGGGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACATCTATT 661 TCCCGTCATA TGACAAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt