Construct: ORF TRCN0000472139
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014890.1_s317c1
- Derived from:
- ccsbBroadEn_11792
- DNA Barcode:
- CTGTATTCCGTGGTCCGCTTAAGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C2CD2 (25966)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472139
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25966 | C2CD2 | C2 calcium dependent domain... | NM_199050.3 | 69.7% | 66.7% | (many diffs) |
| 2 | human | 25966 | C2CD2 | C2 calcium dependent domain... | XM_005261109.4 | 69.7% | 66.7% | (many diffs) |
| 3 | human | 25966 | C2CD2 | C2 calcium dependent domain... | XM_011529524.3 | 66.2% | 65.8% | (many diffs) |
| 4 | human | 25966 | C2CD2 | C2 calcium dependent domain... | XM_011529523.2 | 61.3% | 58.7% | (many diffs) |
| 5 | human | 25966 | C2CD2 | C2 calcium dependent domain... | XM_017028315.1 | 60.2% | 57.3% | (many diffs) |
| 6 | human | 25966 | C2CD2 | C2 calcium dependent domain... | NM_015500.2 | 54.3% | 52.1% | (many diffs) |
| 7 | human | 25966 | C2CD2 | C2 calcium dependent domain... | XM_011529522.2 | 53.4% | 51.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1230
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gctctcccct ttccatttac agctggagtt ccacatgaag gagaagagag 121 aggacctcca gattagctgg tctttcatca gtgtgccgga aatggccgtt aatatccagc 181 ccaaagcact gggggaggac caggtggctg agacaagtgc gatgtctgac gttctcaagg 241 acatcttgaa gcatttggct ggttctgcct ctccatcagt ggttctgatt accaagccca 301 cgactgtaaa ggaagctcag aacttacagt gtgctgcatc tactgctcag gaatcctgtc 361 ctcctaaacc tccaagggct cacgagctga agctactggt gaggaacatc cacgtcttgc 421 tgctcagcga gcccggtgcc tcaggccaca ttaatgcagt gtgcgtcgtg cagctgaacg 481 atcctgttca gaggttctcc agcaccctga cgaaaaacac tccggacctc atgtgggaag 541 aggaattcac cttcgagttg aatgccaagt cgaaggagtt acacctgcag atttcagagg 601 ctgggcgatc ctcagaaggt ctgctggcga cggcgacagt tcctctggac ttatttaaga 661 agcagccttc tgggccacag agcttcacgc tgaccagcgg gtctgcctgc ggcagctcag 721 tgctgggctc ggtcacggca gagttctctt acatggaacc tggtgaattg aaatcctggc 781 ccaTCCCTCC CCCTGTTCCT GCTGCAAAAA TAGAAAAGGA CCGCACGGTG ATGCCCTGTG 841 GGACTGTGGT CACTACTGTC ACTGCTGTGA AGACCAAGCC TCGCGTCGAC GTGGGGAGGG 901 CGTCCCCGCT GAGCTCTGAT TCTCCGGTGA AGACTCCCAT CAAGGTGAAG GTGATCGAGA 961 AGGACATCTC TGTCCAGGCC ATCGCCTGCC GCAGCGCCCC CGTCAGCAAA ACACTCTCTT 1021 CTTCAGACAC AGAATTGTTG GTGTTGAATG GTTCGGATCC AGTGGCTGAA GTGGCCATTC 1081 GACAGCTCAG TGAATCTTCA AAGCTGAAAC TCAAGTCGCC ACGGAAGAAA AGCACTATTA 1141 TCATATCAGG GATCTCCAAG GTACCATCGT GGTGGCTGGG GAGGTGTTCA TCTGGTCATC 1201 TGTCAGTGAC CTGCTGCTCC TCTGCCCTTT GCCCAACTTT CTTGTACAAA GTGGTTGATA 1261 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1321 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACTGTA 1381 TTCCGTGGTC CGCTTAAGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1441 tgaaagatt