Construct: ORF TRCN0000472165
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012995.1_s317c1
- Derived from:
- ccsbBroadEn_08361
- DNA Barcode:
- TTTCCAACTAAGGAATAGGGTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FAM3B (54097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472165
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54097 | FAM3B | FAM3 metabolism regulating ... | NM_058186.4 | 99.8% | 100% | 396T>C |
| 2 | human | 54097 | FAM3B | FAM3 metabolism regulating ... | XM_011529649.2 | 92.8% | 92.7% | (many diffs) |
| 3 | human | 54097 | FAM3B | FAM3 metabolism regulating ... | NM_206964.2 | 78.7% | 77.8% | (many diffs) |
| 4 | human | 54097 | FAM3B | FAM3 metabolism regulating ... | XM_011529648.2 | 74.5% | 74.2% | (many diffs) |
| 5 | human | 54097 | FAM3B | FAM3 metabolism regulating ... | XR_937526.2 | 44.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 771
- ORF length:
- 705
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg cccattggct ggtggcctgc tcaaggtggt gttcgtggtc ttcgcctcct 121 tgtgtgcctg gtattcgggg tacctgctcg cagagctcat tccagatgca cccctgtcca 181 gtgctgccta tagcatccgc agcatcgggg agaggcctgt cctcaaagct ccagtcccca 241 aaaggcaaaa atgtgaccac tggactccct gcccatctga cacctatgcc tacaggttac 301 tcagcggagg tggcagaagc aagtacgcca AAATCTGCTT TGAGGATAAC CTACTTATGG 361 GAGAACAGCT GGGAAATGTT GCCAGAGGAA TAAACATTGC CATTGTCAAC TATGTAACTG 421 GGAATGTGAC AGCAACACGA TGTTTTGATA TGTATGAAGG CGATAACTCT GGACCGATGA 481 CAAAGTTTAT TCAGAGTGCT GCTCCAAAAT CCCTGCTCTT CATGGTGACC TATGACGACG 541 GAAGCACAAG ACTGAATAAC GATGCCAAGA ATGCCATAGA AGCACTTGGA AGTAAAGAAA 601 TCAGGAACAT GAAATTCAGG TCTAGCTGGG TATTTATTGC AGCAAAAGGC TTGGAACTCC 661 CTTCCGAAAT TCAGAGAGAA AAGATCAACC ACTCTGATGC TAAGAACAAC AGATATTCTG 721 GCTGGCCTGC AGAGATCCAG ATAGAAGGCT GCATACCCAA AGAACGAAGC TGCCCAACTT 781 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 841 CTACGTAGTA ATGAACTAGC CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 901 CTTGTGGAAA GGACGATTTC CAACTAAGGA ATAGGGTCGG ACGCGTTAAG TCgacaatca 961 acctctggat tacaaaattt gtgaaagatt