Transcript: Human NM_058186.4

Homo sapiens FAM3 metabolism regulating signaling molecule B (FAM3B), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-22
Taxon:
Homo sapiens (human)
Gene:
FAM3B (54097)
Length:
1317
CDS:
80..787

Additional Resources:

NCBI RefSeq record:
NM_058186.4
NBCI Gene record:
FAM3B (54097)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_058186.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000146983 CAAATCTTGGTACGCAGTATT pLKO.1 932 3UTR 100% 13.200 18.480 N FAM3B n/a
2 TRCN0000372167 TTGGAACTCCCTTCCGAAATT pLKO_005 665 CDS 100% 13.200 18.480 N FAM3B n/a
3 TRCN0000148323 CAACCACTCTGATGCTAAGAA pLKO.1 700 CDS 100% 5.625 7.875 N FAM3B n/a
4 TRCN0000149825 CCACTCTGATGCTAAGAACAA pLKO.1 703 CDS 100% 4.950 6.930 N FAM3B n/a
5 TRCN0000372168 TAAATCCAACAGCCCATATTT pLKO_005 861 3UTR 100% 15.000 12.000 N FAM3B n/a
6 TRCN0000372223 TCAGGTCTAGCTGGGTATTTA pLKO_005 630 CDS 100% 15.000 12.000 N FAM3B n/a
7 TRCN0000147938 GCTTTGAGGATAACCTACTTA pLKO.1 351 CDS 100% 5.625 3.938 N FAM3B n/a
8 TRCN0000148095 GTCAACTATGTAACTGGGAAT pLKO.1 419 CDS 100% 4.050 2.835 N FAM3B n/a
9 TRCN0000147886 GAAGATGTCAATTAGCAGGAA pLKO.1 976 3UTR 100% 2.640 1.848 N FAM3B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_058186.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08361 pDONR223 100% 99.8% 100% None 396T>C n/a
2 ccsbBroad304_08361 pLX_304 0% 99.8% 100% V5 396T>C n/a
3 TRCN0000472165 TTTCCAACTAAGGAATAGGGTCGG pLX_317 62.2% 99.8% 100% V5 396T>C n/a
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