Construct: ORF TRCN0000472185
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008476.1_s317c1
- Derived from:
- ccsbBroadEn_15856
- DNA Barcode:
- TAAAGCCGATCGTCAGATGGCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf78 (51759)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472185
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51759 | C9orf78 | chromosome 9 open reading f... | NM_016520.3 | 99.6% | 99.3% | 39C>T;415C>T;608A>G |
| 2 | human | 51759 | C9orf78 | chromosome 9 open reading f... | XM_011518780.3 | 92.7% | 92.3% | (many diffs) |
| 3 | mouse | 227707 | BC005624 | cDNA sequence BC005624 | NM_144885.2 | 87.2% | 95.1% | (many diffs) |
| 4 | mouse | 227707 | BC005624 | cDNA sequence BC005624 | XM_006497943.3 | 74% | 80.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 933
- ORF length:
- 867
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggtcgtccgg aagattttcc gtcgccgccg gggtgactcg gagtcagagg 121 aagatgagca ggactcagag gaggttcgat taaaactgga agagaccaga gaggtacaga 181 acttgaggaa gaggcccaac ggggtgagtg ctgtggcctt gctggtggga gagaaggtac 241 aagaggagac cactctagtg gatgatccct ttcagatgaa gacaggtggt atggtggata 301 tgaagaaact gaaggaaagg ggcaaagata agatcagtga ggaggaggac ctgcacctgg 361 ggacatcgtt ttctgcagaa accaaccgaa gggatgagga tgcagacatg atgaagtaca 421 ttgagacaga gctaaagaag aggaaaggga tcgtggaaca tgaggaacag aaagttaagt 481 caaagaatgc agaggactgt ctttatgaac ttccagaaaa catccgtgtt tcctcagcaa 541 agaagaccga ggagatgctt tccaaccaga tgctgagtgg cattccTGAG GTGGACCTGG 601 GCATCGATGC TAAAATAAAA AATATCATTT CCACGGAGGA TGCCAAGGCC CGTCTGCTGG 661 CAGAGCAGCA GAGCAAGAAG AAAGACAGCG AGACCTCCTT CGTGCCTACC AACATGGCTG 721 TGAATTATGT GCAGCACAAC AGATTTTATC ATGAGGAGCT CAACGCGCCC ATACGGAGAA 781 ACAAAGAAGA GCCCAAGGCC CGGCCCTTGA GAGTAGGTGA CACGGAGAAG CCAGAGCCTG 841 AGCGGTCCCC TCCTAACCGC AAGCGTCCTG CTAACGAGAA GGCAACTGAT GACTATCATT 901 ATGAGAAGTT CAAGAAAATG AATAGGCGGT ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 961 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1021 GCCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1081 AAGCCGATCG TCAGATGGCC CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1141 ttgtgaaaga tt