Construct: ORF TRCN0000472199
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005198.1_s317c1
- Derived from:
- ccsbBroadEn_13679
- DNA Barcode:
- CCATTTAGCGATTAAATAACAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C16orf74 (404550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472199
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 404550 | C16orf74 | chromosome 16 open reading ... | NM_206967.3 | 84.2% | 84.2% | 1_36del |
| 2 | human | 404550 | C16orf74 | chromosome 16 open reading ... | NR_161454.1 | 21.3% | (many diffs) | |
| 3 | human | 404550 | C16orf74 | chromosome 16 open reading ... | NR_161452.1 | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 320
- ORF end:
- 512
- ORF length:
- 192
- Sequence:
-
1 TCAGCCGTCG CTTCATGTGA CTCCACGGAG TACCGGGCGC CGTCCAGGCA CCTCGATTAG 61 TTCTCGAGCT TTTGGAGTAC GTCGTCTTTA GGTTGGGGGG AGGGGTTTTA TGCGATGGAG 121 TTTCCCCACA CTGAGTGGGT GGAGACTGAA GTTAGGCCAG CTTGGCACTT GATGTAATTC 181 TCCTTGGAAT TTGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 241 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT GATCAACAAG 301 TTTGTACAAA AAAGTTGGCA TGTGTGTCAG CAGCAGCAGC AGCAGCCACG ACGAGGCCCC 361 CGTCCTGAAC GACAAGCACC TGGACGTGCC CGACATCATC ATCACGCCCC CCACCCCCAC 421 GGGCATGATG CTGCCGAGGG ACTTGGGGAG CACAGTCTGG CTGGATGAGA CAGGGTCGTG 481 CCCAGATGAT GGAGAAATCG ACCCAGAAGC CTGCCCAACT TTCTTGTACA AAGTGGTTGA 541 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 601 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCA 661 TTTAGCGATT AAATAACAGA AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 721 tgtgaaagat t