Construct: ORF TRCN0000472219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001044.1_s317c1
- Derived from:
- ccsbBroadEn_02683
- DNA Barcode:
- GTGAGGCCCGCAGTACTAACCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COG2 (22796)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472219
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22796 | COG2 | component of oligomeric gol... | NM_007357.3 | 100% | 100% | |
| 2 | human | 22796 | COG2 | component of oligomeric gol... | NM_001145036.2 | 99.8% | 99.8% | 1648_1649insCAG |
| 3 | mouse | 76332 | Cog2 | component of oligomeric gol... | NM_029746.3 | 80.5% | 85.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2280
- ORF length:
- 2214
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaaaagtagg atgaacctgc ccaaggggcc ggacacgctc tgcttcgaca 121 aggacgagtt catgaaggaa gatttcgatg tcgatcattt tgtgtctgac tgtaggaagc 181 gggtccagct ggaagaactg agagatgacc tggagctcta ctataaactt cttaaaacag 241 ccatggtcga actcatcaac aaggattatg cagattttgt caatctttca acaaacttgg 301 ttggcatgga caaagccctc aaccagcttt ctgtgccttt gggacaatta cgagaagagg 361 ttctgagcct tagatcgtct gtcagtgaag gaattcgggc agttgatgaa cgaatgtcta 421 aacaagagga cattaggaaa aaaaagatgt gtgtattgag gcttatacaa gttattcggt 481 cagttgagaa aattgaaaaa atcttaaact ctcaaagttc taaagaaacc tctgcactag 541 aagcaagcag cccccttttg actggacaaa ttttggagag aattgccaca gaatttaatc 601 agttacagtt tcatgctgtt caaagcaaag gcatgcctct tttggacaaa gtaagaccgc 661 gtatagctgg cattacagcc atgttacagc agtcactgga aggtctccta ttagaaggcc 721 ttcagacgtc tgacgtcgat ataatacggc actgcttgcg gacttacgcc acgattgaca 781 agacacggga cgcggaggcc ttagttggcc aagtactagt gaaaccatac atagacgagg 841 tgattataga gcagtttgtt gaatctcatc ccaatggcct tcaggtcatg tataataaac 901 tcctggagtt tgttcctcac cattgccgcc ttcttcgaga agtcacagga ggtgccatct 961 ccagtgaaaa aggcaatact gttcctggat atgacttttt ggtgaattct gtttggccac 1021 aaatagtaca aggattagaa gaaaagttac cctcgctttt taatcctggg aatcccgatg 1081 catttcatga gaaatatacc ataagtatgg attttgtcag aagattggaa cggcagtgtg 1141 gatcacaggc tagtgtaaag agattaagag cccatcctgc ctatcacagc ttcaataaga 1201 agtggaactt gcctgtttat tttcaaataa gatttagaga aatagcggga tccttagaag 1261 cagcacttac agatgtcctg gaagatgccc cagctgaaag tccgtattgc cttttggctt 1321 ctcatagaac ttggagcagc cttaggaggt gttggtcaga tgagatgttc ttgccattac 1381 tggtgcatcg cctgtggaga ctcactctgc agattttggc acgatactct gtgtttgtca 1441 atgagctttc actcaggccc atttctaatg aaagtcccaa ggagatcaag aaacctttgg 1501 taactggtag caaagaacct tccatcaccc aaggaaacac tgaagaccaa ggaagtggtc 1561 cttcggaaac aaagcctgtg gtttccattt cccgcactca gctcgtgtat gtggttgcag 1621 acctggacaa gcttcaggag cagcttccag aactcttgga aataatcaag ccaaaacttg 1681 aaatgattgg ctttaagaat ttttcttcta tctcagcagc cctggaggac tcccagagct 1741 ctttttcagc ctgtgtgccc tccttgagta gcaagatcat ccaggattta agtgactctt 1801 gcttcggttt cctaaaaagc gccctggagg ttcccaggct ttaccgaaga accaataagg 1861 aggtcccaac cacagcttcc tcctatgtgg acagtgcTCT GAAGCCCTTA TTCCAGCTTC 1921 AGAGCGGACA CAAGGATAAG CTCAAACAAG CAATAATTCA GCAGTGGCTA GAAGGCACTC 1981 TCAGTGAAAG CACTCATAAG TACTATGAAA CCGTGTCAGA TGTATTAAAC TCTGTGAAGA 2041 AGATGGAAGA GAGCCTGAAA AGGCTGAAAC AAGCCAGAAA AACCACTCCC GCCAACCCCG 2101 TCGGTCCCAG TGGTGGCATG AGCGACGACG ACAAAATCAG GCTGCAGTTG GCCCTAGATG 2161 TTGAGTACTT GGGAGAGCAG ATACAAAAGT TGGGACTACA AGCAAGTGAC ATAAAAAGCT 2221 TCTCAGCTCT CGCAGAGCTT GTTGCTGCTG CCAAGGACCA GGCAACAGCA GAGCAGCCTT 2281 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 2341 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 2401 TTTATATATC TTGTGGAAAG GACGAGTGAG GCCCGCAGTA CTAACCTTAA CGCGTTAAGT 2461 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt