Construct: ORF TRCN0000472219

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001044.1_s317c1
Derived from:
ccsbBroadEn_02683
DNA Barcode:
GTGAGGCCCGCAGTACTAACCTTA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
COG2 (22796)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472219
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 22796 COG2 component of oligomeric gol... NM_007357.3 100% 100%
2 human 22796 COG2 component of oligomeric gol... NM_001145036.2 99.8% 99.8% 1648_1649insCAG
3 mouse 76332 Cog2 component of oligomeric gol... NM_029746.3 80.5% 85.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2280
ORF length:
2214
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gaaaagtagg atgaacctgc ccaaggggcc ggacacgctc tgcttcgaca
121aggacgagtt catgaaggaa gatttcgatg tcgatcattt tgtgtctgac tgtaggaagc
181gggtccagct ggaagaactg agagatgacc tggagctcta ctataaactt cttaaaacag
241ccatggtcga actcatcaac aaggattatg cagattttgt caatctttca acaaacttgg
301ttggcatgga caaagccctc aaccagcttt ctgtgccttt gggacaatta cgagaagagg
361ttctgagcct tagatcgtct gtcagtgaag gaattcgggc agttgatgaa cgaatgtcta
421aacaagagga cattaggaaa aaaaagatgt gtgtattgag gcttatacaa gttattcggt
481cagttgagaa aattgaaaaa atcttaaact ctcaaagttc taaagaaacc tctgcactag
541aagcaagcag cccccttttg actggacaaa ttttggagag aattgccaca gaatttaatc
601agttacagtt tcatgctgtt caaagcaaag gcatgcctct tttggacaaa gtaagaccgc
661gtatagctgg cattacagcc atgttacagc agtcactgga aggtctccta ttagaaggcc
721ttcagacgtc tgacgtcgat ataatacggc actgcttgcg gacttacgcc acgattgaca
781agacacggga cgcggaggcc ttagttggcc aagtactagt gaaaccatac atagacgagg
841tgattataga gcagtttgtt gaatctcatc ccaatggcct tcaggtcatg tataataaac
901tcctggagtt tgttcctcac cattgccgcc ttcttcgaga agtcacagga ggtgccatct
961ccagtgaaaa aggcaatact gttcctggat atgacttttt ggtgaattct gtttggccac
1021aaatagtaca aggattagaa gaaaagttac cctcgctttt taatcctggg aatcccgatg
1081catttcatga gaaatatacc ataagtatgg attttgtcag aagattggaa cggcagtgtg
1141gatcacaggc tagtgtaaag agattaagag cccatcctgc ctatcacagc ttcaataaga
1201agtggaactt gcctgtttat tttcaaataa gatttagaga aatagcggga tccttagaag
1261cagcacttac agatgtcctg gaagatgccc cagctgaaag tccgtattgc cttttggctt
1321ctcatagaac ttggagcagc cttaggaggt gttggtcaga tgagatgttc ttgccattac
1381tggtgcatcg cctgtggaga ctcactctgc agattttggc acgatactct gtgtttgtca
1441atgagctttc actcaggccc atttctaatg aaagtcccaa ggagatcaag aaacctttgg
1501taactggtag caaagaacct tccatcaccc aaggaaacac tgaagaccaa ggaagtggtc
1561cttcggaaac aaagcctgtg gtttccattt cccgcactca gctcgtgtat gtggttgcag
1621acctggacaa gcttcaggag cagcttccag aactcttgga aataatcaag ccaaaacttg
1681aaatgattgg ctttaagaat ttttcttcta tctcagcagc cctggaggac tcccagagct
1741ctttttcagc ctgtgtgccc tccttgagta gcaagatcat ccaggattta agtgactctt
1801gcttcggttt cctaaaaagc gccctggagg ttcccaggct ttaccgaaga accaataagg
1861aggtcccaac cacagcttcc tcctatgtgg acagtgcTCT GAAGCCCTTA TTCCAGCTTC
1921AGAGCGGACA CAAGGATAAG CTCAAACAAG CAATAATTCA GCAGTGGCTA GAAGGCACTC
1981TCAGTGAAAG CACTCATAAG TACTATGAAA CCGTGTCAGA TGTATTAAAC TCTGTGAAGA
2041AGATGGAAGA GAGCCTGAAA AGGCTGAAAC AAGCCAGAAA AACCACTCCC GCCAACCCCG
2101TCGGTCCCAG TGGTGGCATG AGCGACGACG ACAAAATCAG GCTGCAGTTG GCCCTAGATG
2161TTGAGTACTT GGGAGAGCAG ATACAAAAGT TGGGACTACA AGCAAGTGAC ATAAAAAGCT
2221TCTCAGCTCT CGCAGAGCTT GTTGCTGCTG CCAAGGACCA GGCAACAGCA GAGCAGCCTT
2281ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
2341GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
2401TTTATATATC TTGTGGAAAG GACGAGTGAG GCCCGCAGTA CTAACCTTAA CGCGTTAAGT
2461Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)