Construct: ORF TRCN0000472222
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013584.1_s317c1
- Derived from:
- ccsbBroadEn_15978
- DNA Barcode:
- ACAAGTTCAGGGGCGGGGAACTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRRG4 (79056)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472222
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | NM_024081.6 | 99.8% | 100% | 534G>A |
| 2 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | XM_006718314.3 | 99.8% | 100% | 534G>A |
| 3 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | XM_017018275.1 | 61.9% | 62% | 1_414del;948G>A |
| 4 | human | 79056 | PRRG4 | proline rich and Gla domain 4 | XM_006718313.3 | 41.1% | 29.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 744
- ORF length:
- 678
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tacgcttctg gttctactca gccaactgcc cacagttacc ctggggtttc 121 ctcattgcgc aagaggtcca aaggcttcta agcatgcggg agaagaagtg tttacatcaa 181 aagaagaagc aaactttttc atacatagac gccttctgta taatagattt gatctggagc 241 tcttcactcc cggcaaccta gaaagagagt gcaaTGAAGA ACTTTGCAAT TATGAGGAAG 301 CCAGAGAGAT TTTTGTGGAT GAAGATAAAA CGATTGCATT TTGGCAGGAA TATTCAGCTA 361 AAGGACCAAC CACAAAATCA GATGGCAACA GAGAGAAAAT AGATGTTATG GGCCTTCTGA 421 CTGGATTAAT TGCTGCTGGA GTATTTTTGG TTATTTTTGG ATTACTTGGC TACTATCTTT 481 GTATCACTAA GTGTAATAGG CTACAACATC CATGCTCTTC AGCCGTCTAT GAAAGGGGGA 541 GGCACACTCC CTCCATCATT TTCAGAAGAC CTGAGGAGGC TGCCTTGTCT CCATTGCCAC 601 CTTCTGTGGA GGATGCAGGA TTACCTTCTT ATGAACAGGC AGTGGCGCTG ACCAGAAAAC 661 ACAGTGTTTC ACCACCACCA CCATATCCTG GGCACACAAA AGGATTTAGG GTATTTAAAA 721 AATCTATGTC TCTCCCATCT CACTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 781 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 841 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA CAAGTTCAGG 901 GGCGGGGAAC TAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 961 att