Construct: ORF TRCN0000472232
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008204.1_s317c1
- Derived from:
- ccsbBroadEn_03931
- DNA Barcode:
- TGTCCATGTAGGTTGACAGGGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBSN (64145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472232
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64145 | RBSN | rabenosyn, RAB effector | NM_001302378.2 | 100% | 100% | |
| 2 | human | 64145 | RBSN | rabenosyn, RAB effector | NM_022340.4 | 100% | 100% | |
| 3 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_005265384.4 | 100% | 100% | |
| 4 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_005265385.4 | 100% | 100% | |
| 5 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_017007023.1 | 100% | 100% | |
| 6 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_017007024.2 | 88.6% | 88.6% | 0_1ins267 |
| 7 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_017007025.2 | 88.6% | 88.6% | 0_1ins267 |
| 8 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_011534001.2 | 88% | 87.7% | (many diffs) |
| 9 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_017007026.1 | 76.4% | 76.4% | 0_1ins555 |
| 10 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_017007027.2 | 69.5% | 65.6% | 0_1ins475;123_124ins242 |
| 11 | human | 64145 | RBSN | rabenosyn, RAB effector | XM_017007028.2 | 69.5% | 65.6% | 0_1ins475;123_124ins242 |
| 12 | mouse | 78287 | Rbsn | rabenosyn, RAB effector | NM_030081.2 | 84.1% | 83.7% | (many diffs) |
| 13 | mouse | 78287 | Rbsn | rabenosyn, RAB effector | XM_006506768.3 | 84.1% | 83.7% | (many diffs) |
| 14 | mouse | 78287 | Rbsn | rabenosyn, RAB effector | XM_006506769.3 | 74.2% | 73.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2421
- ORF length:
- 2352
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttctctg gacgacccag gggaagtgag ggagggcttc ctctgccctc 121 tgtgcctgaa ggatctgcag tctttctatc agcttcactc acattacgag gaagaacact 181 caggggaaga ccgtgatgtc aaagggcaaa ttaaaagtct tgtccagaag gctaaaaaag 241 caaaggacag gttgttgaaa cgagaagggg atgatcgagc agagtcaggg acccaaggat 301 atgagtcttt cagctatgga ggggttgatc cttacatgtg ggaaccccag gagcttggtg 361 ctgtgagaag ccatctttcc gacttcaaaa aacaccgagc tgctagaatt gaccactatg 421 ttgtggaagt caataaacta ataatcaggt tagagaagct cactgcattt gacagaacaa 481 atactgagtc tgcaaagatt cgagcaatag aaaagtctgt ggtgccttgg gtcaacgacc 541 aggatgtccc tttctgtcca gactgtggga ataagttcag catccggaac cgccgccacc 601 actgccgcct ctgcgggtct attatgtgca agaagtgtat ggagctcatc agccttccct 661 tggcaaacaa gctcaccagt gccagcaagg agtccctgag cacccacacc agccccagcc 721 agtcacccaa cagtgtccat ggctcccgcc gaggcagcat cagcagcatg agcagtgtca 781 gctcggtcct ggatgagaag gacgatgacc ggatccgctg ctgtacacac tgcaaggaca 841 cgctgctcaa gagagagcag cagattgatg agaaggagca cacacctgac atcgtgaagc 901 tctacgagaa attacgactt tgcatggaga aagttgacca gaaagctcca gaatacatca 961 ggatggcagc atcattaaat gctggggaga caacctacag tctggaacat gccagtgacc 1021 ttcgagtgga agtgcagaaa gtgtatgagc tgatagacgc tttaagtaag aagatcttaa 1081 ccttgggctt gaaccaggac cctccaccac atccaagcaa tttgcggctg cagagaatga 1141 tcagatactc agctacactt tttgtgcagg aaaagttgct tggtttgatg tcactgccaa 1201 ccaaagaaca gtttgaggaa ctgaaaaaga aaaggaagga ggaaatggag aggaagaggg 1261 ccgtggagag acaagctgcc ctggagtccc agcgaaggct tgaggaaagg cagagtggcc 1321 tggcttctcg agcggccaac ggggaggtgg catctctccg caggggccct gcccccttga 1381 gaaaggctga gggctggctc ccactgtcag gaggtcaggg gcagagtgag gactcagacc 1441 cgctcctcca gcagatccac aacatcacat cattcatcag gcaggccaag gccgcgggcc 1501 gcatggatga agtgcgcact ctgcaggaga acctgcggca gctgcaggac gagtatgacc 1561 agcagcagac agagaaggcc atcgagctgt cccggaggca ggctgaggag gaggacctgc 1621 agcgggaaca gctgcagatg ttgcgtgaac gggagttgga acgagaaagg gagcagtttc 1681 gggtggcatc cctgcacaca cggactcggt ccctggactt cagagaaatc ggcccttttc 1741 agctggagcc cagcagagag cctcgcaccc accttgctta tgctttggat ctaggctctt 1801 ccccagttcc aagcagcaca gctcccaaga ccccttcact tagctcaact caacccacca 1861 gagtgtggtc tgggccccca gccgttggcc aggagcgctt accccagagc agcatgccac 1921 agcaacatga ggggccctcc ttaaacccct ttgatgagga agacctctcc agccccatgg 1981 aagaggccac tactggtcct cctgctgcag gggtttcctt agacccttca gcccgcatcc 2041 tgaaagagta caatcctttc gaggaagagg acgaggagga ggaagcagtg gcagggaaTC 2101 CATTCATTCA GCCAGACAGC CCAGCTCCTA ACCCCTTCAG TGAGGAAGAC GAACATCCCC 2161 AGCAGAGGCT CTCAAGCCCT CTGGTTCCTG GTAACCCCTT TGAGGAACCC ACCTGTATCA 2221 ACCCCTTTGA GATGGACAGT GACAGTGGGC CAGAGGCTGA GGAGCCCATA GAGGAAGAGC 2281 TCCTCCTGCA GCAGATCGAT AACATCAAGG CATACATCTT TGATGCCAAG CAGTGCGGCC 2341 GCCTGGATGA GGTAGAGGTG CTGACAGAGA ATCTGCGGGA GCTGAAGCAC ACCCTGGCCA 2401 AGCAGAAGGG GGGCACTGAC TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2461 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2521 AAAGTATTTG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATGTCC ATGTAGGTTG 2581 ACAGGGCCAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt