Construct: ORF TRCN0000472236
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018142.1_s317c1
- Derived from:
- ccsbBroadEn_04708
- DNA Barcode:
- GAGACACGTGAAATGGTGCCCTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL22RA2 (116379)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472236
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116379 | IL22RA2 | interleukin 22 receptor sub... | NM_181309.2 | 100% | 100% | |
| 2 | human | 116379 | IL22RA2 | interleukin 22 receptor sub... | NM_052962.3 | 87.8% | 87.8% | 198_293del |
| 3 | human | 116379 | IL22RA2 | interleukin 22 receptor sub... | NM_181310.2 | 54.7% | 54.1% | 377_379delGAG;385_388delGGTTinsA;390_391ins309 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 762
- ORF length:
- 693
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gatgcctaaa cattgctttc taggcttcct catcagtttc ttccttactg 121 gtgtagcagg aactcagtca acgcatgagt ctctgaagcc tcagagggta caatttcagt 181 cccgaaattt tcacaacatt ttgcaatggc agcctgggag ggcacttact ggcaacagca 241 gtgtctattt tgtgcagtac aaaatatatg gacagagaca atggaaaaat aaagaagact 301 gttggggtac tcaagaactc tcttgtgacc ttaccagtga aaccTCAGAC ATACAGGAAC 361 CTTATTACGG GAGGGTGAGG GCGGCCTCGG CTGGGAGCTA CTCAGAATGG AGCATGACGC 421 CGCGGTTCAC TCCCTGGTGG GAAACAAAAA TAGATCCTCC AGTCATGAAT ATAACCCAAG 481 TCAATGGCTC TTTGTTGGTA ATTCTCCATG CTCCAAATTT ACCATATAGA TACCAAAAGG 541 AAAAAAATGT ATCTATAGAA GATTACTATG AACTACTATA CCGAGTTTTT ATAATTAACA 601 ATTCACTAGA AAAGGAGCAA AAGGTTTATG AAGGGGCTCA CAGAGCGGTT GAAATTGAAG 661 CTCTAACACC ACACTCCAGC TACTGTGTAG TGGCTGAAAT ATATCAGCCC ATGTTAGACA 721 GAAGAAGTCA GAGAAGTGAA GAGAGATGTG TGGAAATTCC ATTGCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGAGAG ACACGTGAAA TGGTGCCCTG GACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t