Construct: ORF TRCN0000472240
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010954.1_s317c1
- Derived from:
- ccsbBroadEn_03698
- DNA Barcode:
- CGCCTGAGTGAGGTATCTTTGTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCDHGC4 (56098)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472240
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56098 | PCDHGC4 | protocadherin gamma subfami... | NM_032406.1 | 100% | 100% | |
| 2 | human | 56098 | PCDHGC4 | protocadherin gamma subfami... | NM_018928.2 | 89.6% | 88.3% | (many diffs) |
| 3 | mouse | 93707 | Pcdhgc4 | protocadherin gamma subfami... | NM_033582.2 | 82.6% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2682
- ORF length:
- 2613
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gctccgcaag gtgagaagct ggacagaaat ctggcggtgg gctacccttt 121 tgttcctctt ttaccacctg ggttacgttt gtgggcagat ccgctacccg gtcccagagg 181 agtcacagga agggactttt gtagggaatg tcgctcaaga tttcctgctg gatacggaca 241 gtctgtcagc tcgcaggctg caggtcgctg gagaggtgaa ccaaagacac ttccgtgtgg 301 atttggacag cggagccctg ctcatcaaga acccaatcga ccgagaggca ctgtgtgggc 361 tcagtgccag ctgcatcgtg cccctggagt ttgtcaccga aggtcctttg gaaatgtacc 421 gagcagaggt agagatcgta gatgtgaatg atcacgcccc ccgttttccg cggcagcagc 481 tggacttgga aattggggag gcagctcctc caggacagcg tttcccgttg gaaaaggctc 541 aggatgcaga tgtggggagc aattcgatta gcagctatag gctgagctcc aatgaacact 601 ttgcactgga tgtgaagaag cgcagcgacg gcagcctggt cccagagctg ctcctggaga 661 agcctttgga tcgagagaag caatcggact accgcctggt gctgactgct gtcgatggag 721 ggaacccgcc gagatctggc accgcagagc tccgggtatc cgtgctggac gtaaacgaca 781 acgccccagc cttccagcaa tccagctaca ggattagtgt gttggagagc gcaccagcgg 841 gcatggtgct catccagctc aatgcctcag acccggacct gggtcccagt ggtaacgtca 901 ccttttattt cagtggtcat acccctgatc gtgtaagaaa cctctttagc ctgcacccca 961 ctactggaaa gcttactctt ttggggcccc tagactttga gagtgagaat tactatgaat 1021 ttgatgtgcg ggctcgcgat gggggttctc cagccatgga gcaacattgc agccttcgag 1081 tggatctgct ggacgtaaat gacaatgccc cttacatcac agtgacctca gagcttggaa 1141 ccctccccga gagtgcagaa cctggcactg tggtggcact tatcagtgtg caggatccag 1201 actcagggtc aaacggagat gtgagcctcc gcattcctga ccacttgcca tttgccctca 1261 agtctgcctt caggaaccag ttctccctgg tgactgctgg acccttggat cgagaggcca 1321 aatctagcta tgacatcatg gtcactgctt ctgatgctgg gaaccctcct ctcagtaccc 1381 acagaactat tttcctcaat atttcagatg tgaatgataa tccaccctct ttctttcaga 1441 ggtcacatga ggtgtttgtt cctgagaaca atcgcccagg ggacctgctt tgctcccttg 1501 cagcctctga cccagactct ggcttgaatg cgcttatctc ctactcactc ctggagccca 1561 ggaatcgaga tgtatcagct tcctctttca tctctctgaa cccccagaca ggagctgttc 1621 atgctactcg atcctttgac tatgagcaaa cccagacact gcagtttgag gtgcaggccc 1681 gggatcgggg caacccaccc cttagcagca ctgtaacagt tcgtctattt gtgctggacc 1741 tcaatgacaa tgctccagct gtgctccgtc ctcgggcccg gcctggttcc ttatgtcccc 1801 aagcactgcc tccatcagtt ggtgctggcc acctaatcac aaaggtgact gctgtggact 1861 tggattcagg ttacaatgct tgggtttcct atcagctcct ggaggcccca gatcccagcc 1921 tgtttgcagt ctctcgatat gctggggagg tgcggacggc tgttcctatc ccagctgacc 1981 tcccaccaca gaagctggtc attgtggtaa aggatagtgg tagtccacca ctctctacct 2041 ctgttactct cttagtgtcc ttagaggaag acactcatcc agttgtccca gatcttcgag 2101 aatcttcagc tccaagggaa ggagaatctc gtctaaccct ctacttggct gtgtccctag 2161 tggcaatttg ctttgtctcc tttggctcat tcgtggcact actctctaag tgtcttcgtg 2221 gggcagcctg tggagtcaca tgctttcctg ctggcacctg tgcctgtctc accagatctc 2281 gaaggaggga ggggcttccc ccttccaatg ggaTCCTCCG AATCCAGCTA GGGTCAGATG 2341 ACCCTATCAA GTTTGTTGAT GTGGGAGGCC ACTCTCATGG CTGTACACCC TTGGCTTCTG 2401 CACCCACTCG GAGTGATAGC TTCATGATGG TGAAGTCACC CAGTGCACCT ATGGCAGGGG 2461 AGCCTGTTCG CCCAAGCTGC CCACCCTCTG ATCTTCTCTA TGGGCTAGAG GTGAGACCTT 2521 TGCAGGCTCA ACAAATGCTT GAGGGTTATT CTGATCCAGG CATATGGCTA GGCCATGTCC 2581 TAGAGAGTAC TGGCCTCTCA GTAAGTGCCC ATAGTGATGT CACCATTTTT GTAAGAGGTA 2641 ACTATGTGGT AGACGCTGTG CTTTGTAACT GTTTCGTGAA TTTGCCAACT TTCTTGTACA 2701 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2761 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2821 AGGACGACGC CTGAGTGAGG TATCTTTGTA CACGCGTTAA GTCgacaatc aacctctgga 2881 ttacaaaatt tgtgaaagat t