Construct: ORF TRCN0000472255
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007282.1_s317c1
- Derived from:
- ccsbBroadEn_02628
- DNA Barcode:
- TAGAAGTGTAACCCAACCAATGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLAAT3 (11145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472255
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11145 | PLAAT3 | phospholipase A and acyltra... | NM_001128203.1 | 100% | 100% | |
| 2 | human | 11145 | PLAAT3 | phospholipase A and acyltra... | NM_007069.3 | 100% | 100% | |
| 3 | human | 11145 | PLAAT3 | phospholipase A and acyltra... | XM_006718426.1 | 100% | 100% | |
| 4 | human | 11145 | PLAAT3 | phospholipase A and acyltra... | XM_011544741.1 | 87.4% | 89.2% | 1_56del;57_58insTGCGTGCGCCC;60A>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg tgcgcccatt ccagagccta agcctggaga cctgattgag atttttcgcc 121 ctttctacag acactgggcc atctatgttg gcgatggata tgtggttcat ctggccccTC 181 CAAGTGAGGT CGCAGGAGCT GGTGCAGCCA GTGTCATGTC CGCCCTGACT GACAAGGCCA 241 TCGTGAAGAA GGAATTGCTG TATGATGTGG CCGGGAGTGA CAAGTACCAG GTCAACAACA 301 AACATGATGA CAAGTACTCG CCGCTGCCCT GCAGCAAAAT CATCCAGCGG GCGGAGGAGC 361 TGGTGGGGCA GGAGGTGCTC TACAAGCTGA CCAGTGAGAA CTGCGAGCAC TTTGTGAATG 421 AGCTGCGCTA TGGAGTCGCC CGCAGTGACC AGGTCAGAGA TGTCATCATC GCTGCAAGCG 481 TTGCAGGAAT GGGCTTGGCA GCCATGAGCC TTATTGGAGT CATGTTCTCA AGAAACAAGC 541 GACAAAAGCA ATACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATAG AAGTGTAACC CAACCAATGA 721 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t