Construct: ORF TRCN0000472256
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009998.1_s317c1
- Derived from:
- ccsbBroadEn_11781
- DNA Barcode:
- TCACCCTCTCAATAATTGGGGCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC28A (25901)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472256
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25901 | CCDC28A | coiled-coil domain containi... | NM_015439.2 | 58.9% | 58.3% | 1_337del;342_343insA |
| 2 | human | 25901 | CCDC28A | coiled-coil domain containi... | XM_011535728.3 | 31.1% | 30.6% | 1_337del;342_343insA;594_594delTins229 |
| 3 | mouse | 215814 | Ccdc28a | coiled-coil domain containi... | NM_144820.3 | 75.4% | 82% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 552
- ORF length:
- 486
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc aaaaaaaaat gccattccag tgagtaaaag cacagggttt tcaaatcctg 121 catcacagtc aacttcacag cgaccaaagt taaaaagagt gatgaaagaa aagaccaaac 181 cTCAGGGTGG AGAGGGCAAA GGCGCTCAGT CAACTCCGAT CCAGCACTCC TTCCTCACTG 241 ATGTCTCAGA TGTTCAGGAG ATGGAGAGAG GGCTGCTCAG TCTTTTGAAT GATTTCCACT 301 CTGGAAAACT TCAAGCATTT GGAAATGAAT GTTCCATTGA ACAGATGGAA CATGTTCGGG 361 GAATGCAGGA GAAATTAGCT CGCTTGAATT TGGAGCTCTA TGGGGAGTTA GAGGAACTTC 421 CTGAGGATAA GAGAAAAACA GCCAGTGACT CCAATCTGGA TAGGCTTCTG TCAGATTTAG 481 AAGAATTGAA TTCTTCCATA CAAAAACTCC ATTTGGCAGA TGCACAAGAT GTTCCAAATA 541 CTTCTGCTAG CTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 601 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 661 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATCA CCCTCTCAAT AATTGGGGCG 721 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t