Construct: ORF TRCN0000472258
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013478.1_s317c1
- Derived from:
- ccsbBroadEn_07542
- DNA Barcode:
- GACCTTAATTGACCACTTCTTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCF2 (10061)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472258
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10061 | ABCF2 | ATP binding cassette subfam... | NM_007189.3 | 99.8% | 100% | 279T>C;939C>T |
| 2 | human | 114483834 | LOC114483834 | ABCF2-H2B readthrough | NM_005692.4 | 98.1% | 98.2% | 279T>C;939C>T;1870_1902del |
| 3 | human | 114483834 | LOC114483834 | ABCF2-H2B readthrough | NR_160983.1 | 80.6% | (many diffs) | |
| 4 | mouse | 27407 | Abcf2 | ATP-binding cassette, sub-f... | NM_001190443.1 | 91.2% | 98.2% | (many diffs) |
| 5 | mouse | 27407 | Abcf2 | ATP-binding cassette, sub-f... | NM_013853.2 | 91.1% | 98.2% | (many diffs) |
| 6 | mouse | 27407 | Abcf2 | ATP-binding cassette, sub-f... | XM_006535726.3 | 91.1% | 98.2% | (many diffs) |
| 7 | mouse | 27407 | Abcf2 | ATP-binding cassette, sub-f... | XM_006535727.3 | 91.1% | 98.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1935
- ORF length:
- 1869
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctccgacctg gccaagaaga aggcagccaa aaagaaggag gctgccaaag 121 ctcgacagcg gcccagaaaa ggacatgaag aaaatggaga tgttgtcaca gaaccacagg 181 tggcagagaa gaatgaggcc aatggcagag agaccacaga agtagatttg ctgaccaagg 241 agctagagga ctttgagatg aagaaagctg ctgctcgagc tgtcactggc gtcctggcct 301 ctcaccccaa cagtactgat gttcacatca tcaacctctc actcaccttt catggtcaag 361 agctgctcag tgacaccaaa ctggaattaa actcaggccg tcgttatggc ctcattggtt 421 taaatggaat tggaaagtcc atgctgctct ctgctattgg gaagcgtgaa gtgcccatcc 481 ctgagcacat cgacatctac catctgactc gagagatgcc ccctagtgac aagacaccct 541 tgcattgtgt gatggaagtc gacacagagc gggccatgct ggagaaagag gcagagcggc 601 tggctcatga ggatgcggag tgtgagaagc tcatggagct ctacgagcgc ctggaggagc 661 tggatgccga caaggcagag atgagggcct cgcggatctt gcatggactg ggtttcacac 721 ctgccatgca gcgcaagaag ctaaaagact tcagtggggg ctggaggatg agggttgccc 781 ttgccagagc cctctttatt cggcccttca tgctgctcct ggatgagcct accaaccacc 841 tggacctaga tgcttgcgtg tggttggaag aagaactaaa aacttttaag cgcatcttgg 901 tcctcgtctc ccattcccag gattttctga atggtgtctg taccaatatc attcacatgc 961 acaacaagaa actgaagtat tatacgggta attatgatca gtatgtgaag acgcggctag 1021 agctggagga gaaccagatg aagaggtttc actgggagca agatcagatt gcacacatga 1081 agaactacat tgcgaggttt ggtcatggca gtgccaagct ggcccggcag gcccagagca 1141 aggagaagac gctacagaaa atgatggcat caggactgac agagagggtc gtgagcgata 1201 agacactgtc attttatttc ccaccatgtg gcaagatccc tccacctgtc attatggtgc 1261 aaaatgtgag cttcaagtat acaaaagatg ggccttgcat ctacaataat ctagaatttg 1321 gaattgacct tgacacacga gtggctctgg tagggcccaa tggagcaggg aagtcaactc 1381 ttctgaagct gctaactgga gagctactac ccacagatgg catgatccga aaacactctc 1441 atgtcaagat agggcgttac catcagcatt tacaagagca gctggactta gatctctcac 1501 ctttggagta catgatgaag tgctacccag agatcaagga gaaggaagaa atgaggaaga 1561 tcattgggcg atacggtctc actgggaaac aacaggtgag cccaatccgg aacttgtcag 1621 acgggCAGAA GTGCCGAGTG TGTCTGGCCT GGCTGGCCTG GCAGAACCCC CACATGCTCT 1681 TCCTGGATGA ACCCACCAAT CACCTGGATA TCGAGACCAT CGACGCCCTG GCAGATGCCA 1741 TCAATGAGTT TGAGGGTGGT ATGATGCTGG TCAGCCATGA CTTCAGACTC ATTCAGCAGG 1801 TTGCACAGGA AATTTGGGTC TGTGAGAAGC AGACAATCAC CAAGTGGCCT GGAGACATCC 1861 TGGCTTACAA GGAGCACCTC AAGTCCAAGC TGGTGGATGA GGAGCCCCAG CTCACCAAGA 1921 GGACCCACAA CGTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1981 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2041 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACCTTAATT GACCACTTCT 2101 TTTAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt