Construct: ORF TRCN0000472259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017370.1_s317c1
- Derived from:
- ccsbBroadEn_09249
- DNA Barcode:
- CCATGTGCGGCATAGCGTTTGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C1orf94 (84970)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84970 | C1orf94 | chromosome 1 open reading f... | NM_032884.5 | 99.7% | 99.5% | 105G>A;133C>G;336C>G |
| 2 | human | 84970 | C1orf94 | chromosome 1 open reading f... | NM_001134734.2 | 68% | 67.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1290
- ORF length:
- 1224
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgttatcagc agcaggcagg actgtgattc tgccacttct actgtcacag 121 acattctgtg tgccgccgag gtcaagagca gcaaggggac agaggacaga ggccgcatcc 181 taggtgactc caacttggaa gtcagcaagc ttctgtccca gttcccactg aagtccactg 241 agacatccaa ggtccctgac aacaagaatg tgctggacaa gacaagggtc accaaggact 301 tcctacagga caacctgttc agtggccctg gacccaagga gcccacaggg ctgagcccat 361 ttctgctgct gcctccccga cctcctcctg cacgtcctga gaagctccct gagctccctg 421 ctcagaagag gcagctccca gtgtttgcca agatctgttc caagcccaag gctgaccctg 481 ctgtggagag gcaccacttg atggaatgga gccctggcac caaggagcca aaaaagggtc 541 aagggagcct ctttctcagc cagtggcccc agagccagaa ggacgcctgt ggtgaggagg 601 gttgctgtga cgcagtgggc accgcatcac tgaccctgcc gcccaagaaa cctacatgtc 661 cagccgagaa gaacttgctc tatgagttcc ttggggccac caagaaccca agcgggcagc 721 cgagacttcg aaacaaagtg gaagtggatg ggccggagct gaaatttaac gcacctgtga 781 cggttgctga caagaacaac ccgaagtaca cagggaatgt tttcactcca cactttccta 841 cagccatgac ctcagcaacc ctgaaccagc cactctggct caacctgaac tatccacctc 901 caccagtgtt cacgaatcac tctaccttct tgcagtatca gggCCTGTAC CCACAGCAGG 961 CAGCGAGGAT GCCCTATCAG CAGGCTTTGC ACCCGCAGCT GGGATGTTAC TCCCAACAGG 1021 TGATGCCATA CAACCCACAG CAGATGGGAC AGCAGATCTT CCGCTCTTCC TACACCCCTC 1081 TGCTGAGCTA CATCCCTTTT GTCCAGCCCA ATTATCCCTA CCCTCAGAGG ACACCTCCAA 1141 AGATGTCTGC CAACCCCCGA GACCCTCCCC TAATGGCAGG AGATGGACCG CAGTACCTCT 1201 TTCCCCAAGG ATATGGGTTC GGCTCGACAT CCGGAGGGCC CTTGATGCAC AGCCCCTATT 1261 TTTCTTCCAG TGGGAATGGC ATAAACTTTT ACCCAACTTT CTTGTACAAA GTGGTTGATA 1321 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1381 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACCATG 1441 TGCGGCATAG CGTTTGCCGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1501 tgaaagatt