Construct: ORF TRCN0000472267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008188.1_s317c1
- Derived from:
- ccsbBroadEn_02819
- DNA Barcode:
- CCCAGACGAACGATGGATGAGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LY96 (23643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472267
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23643 | LY96 | lymphocyte antigen 96 | NM_015364.5 | 99.7% | 99.3% | 166A>G |
| 2 | human | 23643 | LY96 | lymphocyte antigen 96 | XM_011517508.2 | 82.1% | 77.1% | (many diffs) |
| 3 | human | 23643 | LY96 | lymphocyte antigen 96 | NM_001195797.1 | 81.2% | 80.6% | 112_113ins90 |
| 4 | human | 23643 | LY96 | lymphocyte antigen 96 | XM_017013299.1 | 77.7% | 69% | (many diffs) |
| 5 | human | 23643 | LY96 | lymphocyte antigen 96 | XM_017013300.1 | 62.4% | 53.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 546
- ORF length:
- 480
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt accatttctg tttttttcca ccctgttttc ttccatattt actgaagctc 121 agaagcagta ttgggtctgc aactcatccg atgcaagtat ttcatacacc TACTGTGATA 181 AAATGCAATA CCCAATTTCA ATTAATGTTA ACCCCTGTAT AGAATTGAAA GGATCCAAAG 241 GATTATTGCA CATTTTCTAC ATTCCAAGGA GAGATTTAAA GCAATTATAT TTCAATCTCT 301 ATATAACTGT CAACACCATG AATCTTCCAA AGCGCAAAGA AGTTATTTGC CGAGGATCTG 361 ATGACGATTA CTCTTTTTGC AGAGCTCTGA AGGGAGAGAC TGTGAATACA ACAATATCAT 421 TCTCCTTCAA GGGAATAAAA TTTTCTAAGG GAAAATACAA ATGTGTTGTT GAAGCTATTT 481 CTGGGAGCCC AGAAGAAATG CTCTTTTGCT TGGAGTTTGT CATCCTACAC CAACCTAATT 541 CAAATTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 601 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 661 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCAGACGA ACGATGGATG AGGGAACGCG 721 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt