Construct: ORF TRCN0000472282
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002967.1_s317c1
- Derived from:
- ccsbBroadEn_13822
- DNA Barcode:
- AACCGACGTTCACAAGTCAGGGCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CALM3 (808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472282
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 808 | CALM3 | calmodulin 3 | NM_001329922.1 | 82.3% | 6.6% | (many diffs) |
| 2 | human | 808 | CALM3 | calmodulin 3 | NM_005184.4 | 82.3% | 6.6% | (many diffs) |
| 3 | human | 808 | CALM3 | calmodulin 3 | NM_001329921.1 | 60.2% | 2.8% | (many diffs) |
| 4 | human | 808 | CALM3 | calmodulin 3 | NM_001329923.1 | 60.2% | 2.8% | (many diffs) |
| 5 | human | 808 | CALM3 | calmodulin 3 | NM_001329924.2 | 60.2% | 2.8% | (many diffs) |
| 6 | human | 808 | CALM3 | calmodulin 3 | NM_001329925.2 | 60.2% | 2.8% | (many diffs) |
| 7 | human | 808 | CALM3 | calmodulin 3 | NM_001329926.2 | 60.2% | 2.8% | (many diffs) |
| 8 | mouse | 12315 | Calm3 | calmodulin 3 | NM_007590.3 | 72% | 6.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 171
- ORF length:
- 105
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaccagcga ctgaggagca gattgcagag ttcaaggagg ccTTCTCCCT 121 CTTTGACAAG GATGGAGATG GCACTATCAC CACCAAGGAG TTGGGGACAG TGATGAGATC 181 CCTGGGACAG AACCCCACTG AAGCAGAGCT GCAGGATATG ATCAATGAGG TGGATGCAGA 241 TGGGAACGGG ACCATTGACT TCCCGGAGTT CCTGACCATG ATGGCCAGAA AGATGAAGGA 301 CACAGACAGT GAGGAGGAGA TCCGAGAGGC GTTCCGTGTC TTTGACAAGG ATGGGAATGG 361 CTACATCAGC GCCGCAGAGC TGCGTCACGT AATGACGAAC CTGGGGGAGA AGCTGACCGA 421 TGAGTCTCTC TCCATGCCCC TCATCTCTTC CTTTTGCCCT CGCCTCTTCC ATCCATGTCT 481 TCCAAGGCCT GATGCATTCA TAAGTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 541 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 601 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AAACCGACGT 661 TCACAAGTCA GGGCCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 721 agatt