Construct: ORF TRCN0000472288
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001174.1_s317c1
- Derived from:
- ccsbBroadEn_12665
- DNA Barcode:
- GTTTCAGGACCACAATAAGCCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF556 (80032)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472288
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80032 | ZNF556 | zinc finger protein 556 | NM_001300843.2 | 100% | 100% | |
| 2 | human | 80032 | ZNF556 | zinc finger protein 556 | NM_024967.3 | 99.7% | 99.7% | 313_315delAGC |
| 3 | human | 80032 | ZNF556 | zinc finger protein 556 | NR_145838.2 | 20.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1431
- ORF length:
- 1365
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cacagtggtc tttgaagacg tggttgtgga tttcacgctg gaggagtggg 121 ccttgctgaa tcctgctcag agaaaactct acagagatgt catgctggag accttcaagc 181 acctggcctc agtagataat gaggctcagc ttaaagccag tgggtctatt tctcagcagg 241 atacttctgg agaaaaatta tccctcaaac agaaaataga aaagttcaca agaaagaata 301 tatgggcctc ccttttagga aaaaattggg aagaacatag cgttaaagac aagcacaaca 361 ccaaggagag acatttgaga aatccaaggg tggagagacc atgtaaaagc agtaaaggta 421 ataaacgtgg aagaaccttc agaaagactc gaaattgtaa tcgtcatctg cgcaagaatt 481 gttgtactag tgtaagacgg tacgaatgca gtcagtgtgg aaaactcttc acccattcct 541 catccctgat aaggcacaaa agagctcact ctggacaaaa attatataaa tgtaaggaat 601 gtgggaaagc cttcagtcgc ccttcctacc tacagacgca tgagaaaact cacagtggag 661 agaaacccta tgcctgtcaa tcttgcggga agacatttct tcgttcccac tctctcactg 721 aacatgtaag gactcacact ggagagaaac cctacgaatg tgggcagtgt gggaaaggct 781 tcagttgtcc caaatccttt cgcgcacatg tgatgatgca cgccggaggg agaccgtatg 841 agtgcaagca ctgtgggaaa gccttcaggt gtcagaaatc ctttcgagtc catatgatca 901 tgcacgccgg agggagaccg tatgagtgca agcagtgtgg gaaagccTAC TGCTGGGCAA 961 CATCCTTTCA ACGACACGTG AGAATTCACA ACGGGGAGAA ACCCTATAAG TGTGGAAAAT 1021 GCGGGAAAGC ATTCGGTTGG CCCTCATCCT TACACAAACA CGCGAGAACG CATGCTAAAA 1081 AGAAACCTGT GAGTGGGGGC AGCGTGGGAA AGTCTTCCGC GAGGCCTCGC CCCTCCACAG 1141 ATGTCAAATC ACAAACTAGA GAGAAAGTCT ATAAATGTGA AACGTGTGGG AAAACGTATG 1201 GTTGGTCCTC ATCTTTACAC AAACATGAGA GAAAGCACAC TGGGGAGAAA CCTGTAAATG 1261 CAGCCAGTGT GGGAAAACCT TCAGGCGGGC TTTGCTCTTC CAAAAATGTA AGAACGCAGA 1321 TTGGACAGAA GCCCAGTAAA TGCGAAAAAT GTGGGAAAGC TTTCAGTTGT CCCAAAGCCT 1381 TTCAAGGTCA TGTGAGAAGT CACACAGGAA AGAAATCCTG TACATCTAAG TACCCAACTT 1441 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1501 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1561 CTTGTGGAAA GGACGAGTTT CAGGACCACA ATAAGCCCTC ACGCGTTAAG TCgacaatca 1621 acctctggat tacaaaattt gtgaaagatt