Construct: ORF TRCN0000472290
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008562.1_s317c1
- Derived from:
- ccsbBroadEn_00704
- DNA Barcode:
- CGCTCCGATCACATACCGGGAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GSTZ1 (2954)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472290
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2954 | GSTZ1 | glutathione S-transferase z... | NM_145870.3 | 100% | 100% | |
| 2 | human | 2954 | GSTZ1 | glutathione S-transferase z... | NM_001363703.2 | 98.9% | 98.1% | (many diffs) |
| 3 | human | 2954 | GSTZ1 | glutathione S-transferase z... | NM_145871.3 | 80.5% | 80.5% | 216_217ins126 |
| 4 | human | 2954 | GSTZ1 | glutathione S-transferase z... | XM_011536671.2 | 79.5% | 78.8% | (many diffs) |
| 5 | human | 2954 | GSTZ1 | glutathione S-transferase z... | NM_001312660.2 | 74.5% | 74.5% | 0_1ins165 |
| 6 | human | 2954 | GSTZ1 | glutathione S-transferase z... | XM_024449550.1 | 74.5% | 74.5% | 0_1ins165 |
| 7 | human | 2954 | GSTZ1 | glutathione S-transferase z... | XM_024449551.1 | 74.5% | 74.5% | 0_1ins165 |
| 8 | human | 2954 | GSTZ1 | glutathione S-transferase z... | XM_024449552.1 | 74.5% | 74.5% | 0_1ins165 |
| 9 | human | 2954 | GSTZ1 | glutathione S-transferase z... | XM_011536670.2 | 74.3% | 67.7% | (many diffs) |
| 10 | human | 2954 | GSTZ1 | glutathione S-transferase z... | XM_024449549.1 | 74.3% | 67.7% | (many diffs) |
| 11 | mouse | 14874 | Gstz1 | glutathione transferase zet... | NM_010363.4 | 84.2% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 714
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggcggggaag cccatcctct attcctattt ccgaagctcc tgctcatgga 121 gagttcgaat tgctctggcc ttgaaaggca tcgactacga gacggtgccc atcaatctca 181 taaaggatgg gggccaacag ttttctaagg acttccaggc actgaatcct atgaagcagg 241 tgccaacccT GAAGATTGAT GGAATCACCA TTCACCAGTC ACTGGCCATC ATTGAGTATC 301 TAGAGGAGAT GCGTCCCACT CCGCGACTTC TGCCTCAGGA CCCAAAGAAG AGGGCCAGCG 361 TGCGTATGAT TTCTGACCTC ATCGCTGGTG GCATCCAGCC CCTGCAGAAC CTGTCTGTCC 421 TGAAGCAAGT GGGAGAGGAG ATGCAGCTGA CCTGGGCCCA GAACGCCATC ACTTGTGGCT 481 TTAACGCCCT GGAGCAGATC CTACAGAGCA CAGCGGGCAT ATACTGTGTA GGAGACGAGG 541 TGACCATGGC TGATCTGTGC TTGGTGCCTC AGGTGGCAAA TGCTGAAAGA TTCAAGGTGG 601 ATCTCACCCC CTACCCTACC ATCAGCTCCA TCAACAAGAG GCTGCTGGTC TTGGAGGCCT 661 TCCAGGTGTC TCACCCCTGC CGGCAGCCAG ATACACCCAC TGAGCTGAGG GCCTACCCAA 721 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 781 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 841 TATCTTGTGG AAAGGACGAC GCTCCGATCA CATACCGGGA GTCACGCGTT AAGTCgacaa 901 tcaacctctg gattacaaaa tttgtgaaag att