Construct: ORF TRCN0000472291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000369.1_s317c1
- Derived from:
- ccsbBroadEn_07365
- DNA Barcode:
- CGCACATCAAGTCCCCAGTTCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYNGR1 (9145)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9145 | SYNGR1 | synaptogyrin 1 | NM_145731.3 | 99.8% | 100% | 573G>C |
| 2 | human | 9145 | SYNGR1 | synaptogyrin 1 | NM_145738.2 | 86.6% | 83% | (many diffs) |
| 3 | human | 9145 | SYNGR1 | synaptogyrin 1 | NM_004711.5 | 78.5% | 72.5% | (many diffs) |
| 4 | mouse | 20972 | Syngr1 | synaptogyrin 1 | NM_009303.2 | 90.2% | 94.2% | (many diffs) |
| 5 | mouse | 20972 | Syngr1 | synaptogyrin 1 | NM_207708.1 | 70% | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agggggtgcg tacggagcgg gcaaagccgg gggcgccttc gacccctaca 121 ccctggtccg gcagccgcac accatcctgc gcgtcgtgtc ttggctgttc tccatagtgg 181 tgttcggctc catcgtgaac gagggctacc tcaacagcgc cTCCGAGGGG GAGGAGTTCT 241 GCATCTACAA CCGCAACCCC AACGCCTGCA GCTATGGCGT GGCCGTGGGC GTGCTCGCCT 301 TCCTCACCTG CCTGCTGTAC CTGGCCCTGG ACGTGTACTT CCCGCAGATC AGCAGCGTCA 361 AGGACCGCAA GAAAGCCGTC CTGTCCGACA TCGGTGTCTC GGCCTTCTGG GCTTTCCTCT 421 GGTTCGTGGG ATTCTGCTAC CTGGCCAACC AGTGGCAGGT CTCCAAGCCC AAGGACAACC 481 CACTGAACGA AGGGACGGAC GCAGCCCGGG CCGCCATCGC CTTCTCCTTT TTCTCCATCT 541 TCACCTGGAG CCTGACCGCA GCCCTGGCCG TGCGGAGATT CAAGGACCTA AGCTTCCAGG 601 AGGAGTACAG CACACTGTTC CCTGCCTCGG CACAGCCCTA CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGACGCACA TCAAGTCCCC AGTTCGTGAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt