Construct: ORF TRCN0000472293
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016517.1_s317c1
- Derived from:
- ccsbBroadEn_06236
- DNA Barcode:
- CCTCGTCTTGCTTAAGGATTCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XRCC6 (2547)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472293
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2547 | XRCC6 | X-ray repair cross compleme... | NM_001288976.2 | 99.8% | 100% | 9G>C;1779G>T |
| 2 | human | 2547 | XRCC6 | X-ray repair cross compleme... | NM_001469.5 | 99.8% | 100% | 9G>C;1779G>T |
| 3 | human | 2547 | XRCC6 | X-ray repair cross compleme... | NM_001288977.2 | 93.1% | 93.2% | 9G>C;192_193ins123;1656G>T |
| 4 | human | 2547 | XRCC6 | X-ray repair cross compleme... | NM_001288978.1 | 91.7% | 89.6% | 0_1ins37;44_45ins113;1629G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1893
- ORF length:
- 1827
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aggctgggag tcatattaca aaaccgaggg cgatgaagaa gcagaggaag 121 aacaagaaga gaaccttgaa gcaagtggag actataaata ttcaggaaga gatagtttga 181 tttttttggt tgatgcctcc aaggctatgt ttgaatctca gagtgaagat gagttgacac 241 cttttgacat gagcatccag tgtatccaaa gtgtgtacat cagtaagatc ataagcagtg 301 atcgagatct cttggctgtg gtgttctatg gtaccgagaa agacaaaaat tcagtgaatt 361 ttaaaaatat ttacgtctta caggagctgg ataatccagg tgcaaaacga attctagagc 421 ttgaccagtt taaggggcag cagggacaaa aacgtttcca agacatgatg ggccacggat 481 ctgactactc actcagtgaa gtgctgtggg tctgtgccaa cctctttagt gatgtccaat 541 tcaagatgag tcataagagg atcatgctgt tcaccaatga agacaacccc catggcaatg 601 acagtgccaa agccagccgg gccaggacca aagccggtga tctccgagat acaggcatct 661 tccttgactt gatgcacctg aagaaacctg ggggctttga catatccttg ttctacagag 721 atatcatcag catagcagag gatgaggacc tcagggttca ctttgaggaa tccagcaagc 781 tagaagacct gttgcggaag gttcgcgcca aggagaccag gaagcgagca ctcagcaggt 841 taaagctgaa gctcaacaaa gatatagtga tctctgtggg catttataat ctggtccaga 901 aggctctcaa gcctcctcca ataaagctct atcgggaaac aaatgaacca gtgaaaacca 961 agacccggac ctttaataca agtacaggcg gtttgcttct gcctagcgat accaagaggt 1021 ctcagatcta tgggagtcgt cagattatac tggagaaaga ggaaacagaa gagctaaaac 1081 ggtttgatga tccaggtttg atgctcatgg gtttcaagcc gttggtactg ctgaagaaac 1141 accattacct gaggccctcc ctgttcgtgt acccagagga gtcgctggtg attgggagct 1201 caaccctgtt cagtgctctg ctcatcaagt gtctggagaa ggaggttgca gcattgtgca 1261 gatacacacc ccgcaggaac atccctcctt attttgtggc tttggtgcca caggaagaag 1321 agttggatga ccagaaaatt caggtgactc ctccaggctt ccagctggTC TTTTTACCCT 1381 TTGCTGATGA TAAAAGGAAG ATGCCCTTTA CTGAAAAAAT CATGGCAACT CCAGAGCAGG 1441 TGGGCAAGAT GAAGGCTATC GTTGAGAAGC TTCGCTTCAC ATACAGAAGT GACAGCTTTG 1501 AGAACCCCGT GCTGCAGCAG CACTTCAGGA ACCTGGAGGC CTTGGCCTTG GATTTGATGG 1561 AGCCGGAACA AGCAGTGGAC CTGACATTGC CCAAGGTTGA AGCAATGAAT AAAAGACTGG 1621 GCTCCTTGGT GGATGAGTTT AAGGAGCTTG TTTACCCACC AGATTACAAT CCTGAAGGGA 1681 AAGTTACCAA GAGAAAACAC GATAATGAAG GTTCTGGAAG CAAAAGGCCC AAGGTGGAGT 1741 ATTCAGAAGA GGAGCTGAAG ACCCACATCA GCAAGGGTAC GCTGGGCAAG TTCACTGTGC 1801 CCATGCTGAA AGAGGCCTGC CGGGCTTACG GGCTGAAGAG TGGTCTGAAG AAGCAGGAGC 1861 TGCTGGAAGC CCTCACCAAG CACTTCCAGG ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG 1921 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1981 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 2041 TCGTCTTGCT TAAGGATTCA AAACGCGTTA AGTCgacaat caacctctgg attacaaaat 2101 ttgtgaaaga tt