Construct: ORF TRCN0000472295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004760.1_s317c1
- Derived from:
- ccsbBroadEn_06290
- DNA Barcode:
- AGGGCTGTAGACCAAGTACTTCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNA15 (2769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2769 | GNA15 | G protein subunit alpha 15 | NM_002068.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccgctcgctg acctggcgct gctgcccctg gtgcctgacg gaggatgaga 121 aggccgccgc ccgggtggac caggagatca acaggatcct cttggagcag aagaagcagg 181 accgcgggga gctgaagctg ctgcttttgg gcccaggcga gagcgggaag agcaccttca 241 tcaagcagat gcggatcatc cacggcgccg gctactcgga ggaggagcgc aagggcttcc 301 ggcccctggt ctaccagaac atcttcgtgt ccatgcgggc catgatcgag gccatggagc 361 ggctgcagat tccattcagc aggcccgaga gcaagcacca cgctagcctg gtcatgagcc 421 aggaccccta taaagtgacc acgtttgaga agcgctacgc tgcggccatg cagtggctgt 481 ggagggatgc cggcatccgg gcctgctatg agcgtcggcg ggaattccac ctgctcgatt 541 cagccgtgta ctacctgtcc cacctggagc gcatcaccga ggagggctac gtccccacag 601 ctcaggacgt gctccgcagc cgcatgccca ccactggcat caacgagtac tgcttctccg 661 tgcagaaaac caacctGCGG ATCGTGGACG TCGGGGGCCA GAAGTCAGAG CGTAAGAAAT 721 GGATCCATTG TTTCGAGAAC GTGATCGCCC TCATCTACCT GGCCTCACTG AGTGAATACG 781 ACCAGTGCCT GGAGGAGAAC AACCAGGAGA ACCGCATGAA GGAGAGCCTC GCATTGTTTG 841 GGACTATCCT GGAACTACCC TGGTTCAAAA GCACATCCGT CATCCTCTTT CTCAACAAAA 901 CCGACATCCT GGAGGAGAAA ATCCCCACCT CCCACCTGGC TACCTATTTC CCCAGTTTCC 961 AGGGCCCTAA GCAGGATGCT GAGGCAGCCA AGAGGTTCAT CCTGGACATG TACACGAGGA 1021 TGTACACCGG GTGCGTGGAC GGCCCCGAGG GCAGCAAGAA GGGCGCACGA TCCCGACGCC 1081 TCTTCAGCCA CTACACATGT GCCACAGACA CACAGAACAT CCGCAAGGTC TTCAAGGACG 1141 TGCGGGACTC GGTGCTCGCC CGCTACCTGG ACGAGATCAA CCTGCTGTGC CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAAGGGCTG TAGACCAAGT ACTTCTTACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt