Construct: ORF TRCN0000472296
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011091.1_s317c1
- Derived from:
- ccsbBroadEn_12058
- DNA Barcode:
- ACAAACCAGCGGTAGAGCCCTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECAB2 (54550)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472296
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54550 | NECAB2 | N-terminal EF-hand calcium ... | NM_001329749.2 | 78.8% | 78.8% | 1_192del |
| 2 | human | 54550 | NECAB2 | N-terminal EF-hand calcium ... | NM_019065.2 | 61.9% | 61.9% | 1_441del |
| 3 | human | 54550 | NECAB2 | N-terminal EF-hand calcium ... | XM_017023317.2 | 59.1% | 59.1% | 1_495del |
| 4 | human | 54550 | NECAB2 | N-terminal EF-hand calcium ... | NM_001329748.1 | 57.2% | 57.2% | 1_441del;795_796ins54 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 783
- ORF length:
- 717
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ttataccaag aaggtatatg agggtgggag caacgtggac cagtttgtga 121 cccgcttcct cctgaaggag acggccaatc agatccagtc gctgctgagc tcagtggaga 181 gtgcggtgga ggccatcgag gaacagacca gccagctccg acagaaccac atcaaaccca 241 gccacagcgc ggcacagacc tggtgtggaa gccccactcc cgcctctgcc cccaaccaca 301 agctcatGGC TATGGAACAA GGCAAGACCC TTCCATCTGC CACGGAGGAT GCAAAGGAAG 361 AGGGTCTGGA AGCCCAGATC AGCCGCTTGG CAGAGCTGAT TGGGAGGCTG GAGAGCAAAG 421 CACTGTGGTT CGACCTGCAG CAGCGCCTGT CAGATGAAGA TGGCACCAAC ATGCACCTGC 481 AGCTGGTCCG GCAGGAGATG GCCGTGTGCC CCGAGCAACT GAGCGAGTTT CTGGACTCTC 541 TGCGCCAGTA TCTGCGGGGG ACCACTGGCG TGAGGAACTG CTTCCACATC ACTGCCGTGA 601 GGCTCTCAGA TGGCTTCACC TTTGTCATCT ATGAGTTCTG GGAGACAGAG GAGGCGTGGA 661 AGAGGCACCT GCAGAGCCCC CTGTGTAAGG CGTTCCGGCA CGTCAAGGTG GACACACTGA 721 GCCAGCCTGA GGCCCTCTCC AGGATCTTGG TGCCAGCTGC TTGGTGCACG GTGGGACGGG 781 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 841 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 901 GGCTTTATAT ATCTTGTGGA AAGGACGAAC AAACCAGCGG TAGAGCCCTG TCACGCGTTA 961 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt