Construct: ORF TRCN0000472320
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007470.1_s317c1
- Derived from:
- ccsbBroadEn_10475
- DNA Barcode:
- CGCGGCCCGTGAAAGATAAAAATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- WDR89 (112840)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472320
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112840 | WDR89 | WD repeat domain 89 | NM_001008726.3 | 99.9% | 99.7% | 1159C>T |
| 2 | human | 112840 | WDR89 | WD repeat domain 89 | NM_001258272.2 | 99.9% | 99.7% | 1159C>T |
| 3 | human | 112840 | WDR89 | WD repeat domain 89 | NM_080666.4 | 99.9% | 99.7% | 1159C>T |
| 4 | human | 112840 | WDR89 | WD repeat domain 89 | XM_011536385.3 | 99.9% | 99.7% | 1159C>T |
| 5 | human | 112840 | WDR89 | WD repeat domain 89 | XM_011536383.2 | 90.9% | 90.8% | 1_114del;1273C>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1224
- ORF length:
- 1158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaagattgag gaacaatttg ctaatctgca cattgttaaa tgttccttag 121 gaaccaaaga gcccacttac cttcttggta tagacacatc aaagactgtc caagcaggaa 181 aggaaaactt ggttgctgtt ttatgttcta atggatcaat cagaatatat gataaagaaa 241 ggttaaatgt actacgagaa tttagtggat atcctggact tcttaatgga gtcagatttg 301 caaattcctg tgacagtgta tattcagcat gtactgatgg cactgtgaaa tgctgggatg 361 ctcgagtagc cagagaaaaa cctgttcagc tcttcaaggg ttacccttcc aatattttta 421 tcagttttga tattaattgt aatgatcata ttatttgtgc tggtacagaa aaagttgatg 481 atgatgcatt gttggtgttt tgggatgcaa ggatgaattc tcagaattta tctacaacta 541 aagactcact tggtgcatat tcagagacac atagtgatga tgtcactcaa gtacgtttcc 601 atcccagcaa tcccaacatg gtagtctcag gttcatctga tggcctggta aatgtatttg 661 atattaatat tgataatgag gaggatgcac tggttacaac ctgtaactca atttcatcag 721 taagctgtat tggttggtct gggaaaggtt ataaacagat ttactgcatg acacatgatg 781 aaggatttta ttggtgggat cttaatcatc tggacactga tgaaccagtt acacgtttga 841 acatccagga tgtcagagaa gtagttaaca tgaaagaaga tgctttggac tatttgattg 901 gtggcctata tcatgaaaag acagacacat tgcatgttat tggaggaaca aacaaaggaa 961 ggattcattt gatgaactgc agcatgtcag gactgaccca tgtgactagc cttcagggag 1021 ggcatgctgc tacagtccgt tctttctgtt ggaatgtgca agatgattct ttgttgactg 1081 gaggagaaga tgcacagttg ttactttgga aacctggagc tatagagaag accTTTACAA 1141 AGAAAGAGAG TATGAAAATA GCATCCTCTG TGCACCAACG AGTACGAGTT CATAGTAATG 1201 ATTCTTATAA AAGAAGGAAA AAGTAGAGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACGCGGCCC 1381 GTGAAAGATA AAAATGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1441 aagatt