Construct: ORF TRCN0000472329
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013742.1_s317c1
- Derived from:
- ccsbBroadEn_05512
- DNA Barcode:
- TCTGAAATCTAACTATCACACCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM255B (348013)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472329
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 348013 | TMEM255B | transmembrane protein 255B | NM_182614.4 | 100% | 100% | |
| 2 | human | 348013 | TMEM255B | transmembrane protein 255B | XM_017020560.1 | 93.5% | 93.5% | 189_190ins63 |
| 3 | human | 348013 | TMEM255B | transmembrane protein 255B | XM_005272413.3 | 91.3% | 91.3% | 424_516del |
| 4 | human | 348013 | TMEM255B | transmembrane protein 255B | XM_017020558.1 | 85.5% | 80.3% | 343_410del;492_588del |
| 5 | human | 348013 | TMEM255B | transmembrane protein 255B | NM_001348663.2 | 85.2% | 85.2% | 668_669ins144 |
| 6 | human | 348013 | TMEM255B | transmembrane protein 255B | XM_017020559.1 | 74.5% | 62.7% | (many diffs) |
| 7 | human | 348013 | TMEM255B | transmembrane protein 255B | XM_024449345.1 | 65.4% | 58.8% | (many diffs) |
| 8 | human | 348013 | TMEM255B | transmembrane protein 255B | XM_005272415.3 | 56.5% | 58.5% | 0_1ins368;56_156del |
| 9 | human | 348013 | TMEM255B | transmembrane protein 255B | XM_006719986.2 | 56.5% | 58.5% | 0_1ins368;56_156del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gccgccggtg cccgggcccc tgggcctgct ggaccccgca gaagggcttt 121 cgaggaggaa gaagacgtcg ctctggtttg tggggtctct gctgctggtg tccgtcctca 181 tagtcaccgt cgggctggct gccaccacca ggacggagaa tgtgaccgtt gggggctact 241 acccagggat cattctcggc tttggatctt tcttaggaat tattggcatc aacttggtgg 301 agaatagaag gcaaatgctg gtggcagcga tcgtgtttat cagttttggc gtggtggccg 361 ccttctgctg cgccatcgtg gacggcgtat ttgcagcaca gcacattgaa ccgaggcccc 421 tcaccacggg aagatgccag ttttactcca gtggggtggg gtacttgtac gatgtctacc 481 agacagaggt cacctgtcac tccctggacg gcaagtgcca gctgaaggtg agaagcaaca 541 cctgttactg ctgtgacctc tatgcctgcg ggagcgcaga gccctcgccc gcctactatg 601 agttcatcgg cgtcagcggc tgccaggacg tgctgcacct gtaccgccTG CTCTGGGCCT 661 CTGCAGTTCT GAACGTCCTG GGCCTGTTCC TGGGCATCAT CACCGCCGCC GTCCTGGGGG 721 CCTTCAAGGA CATGGTGCCT CTGTCCCAGC TGGCCTATGG CCCAGCCGTC CCACCACAGA 781 CCCTCTACAA CCCCGCCCAG CAGATCCTGG CCTACGCAGG CTTCCGCCTG ACGCCCGAGC 841 CCGTCCCGAC CTGCTCGTCC TACCCTCTGC CCCTTCAGCC CTGCAGCCGC TTCCCAGTTG 901 CGCCCTCCTC TGCCCTGGCT TCGTCTGAGG ACCTGCAGCC CCCTTCTCCA AGCAGCTCTG 961 GCTCTGGGCT TCCCGGCCAG GCTCCACCGT GCTACGCACC CACCTACTTT CCCCCGGGGG 1021 AGAAGCCACC CCCCTACGCA CCCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT CTGAAATCTA 1201 ACTATCACAC CACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att