Construct: ORF TRCN0000472359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012513.1_s317c1
- Derived from:
- ccsbBroadEn_10001
- DNA Barcode:
- GATGAGAACCGAAAAGTCCGTCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPIHBP1 (338328)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472359
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 338328 | GPIHBP1 | glycosylphosphatidylinosito... | NM_178172.6 | 100% | 100% | |
| 2 | human | 338328 | GPIHBP1 | glycosylphosphatidylinosito... | NM_001301772.2 | 67.9% | 67.9% | 375_376ins177 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggcgctcggg gctgtcctgc ttgccctctt gctgttcggg cggccaggga 121 gagggcagac acagcaggag gaagaggaag aggacgagga ccacgggcca gatgactacg 181 acgaggaaga tgaggatgag gtggaagagg aggagaccaa caggctccct ggtggcagga 241 gcagagtgct gctgcggtgc tacaccTGCA AGTCCCTGCC CAGGGACGAG CGCTGCAACC 301 TGACGCAGAA CTGCTCACAT GGCCAGACCT GCACAACCCT CATTGCCCAC GGGAACACCG 361 AGTCAGGCCT CCTGACCACC CACTCCACGT GGTGCACAGA CAGCTGCCAG CCCATCACCA 421 AGACGGTGGA GGGGACCCAG GTGACCATGA CCTGCTGCCA GTCCAGCCTG TGCAATGTCC 481 CACCCTGGCA AAGCTCCCGA GTCCAGGACC CAACAGGCAA GGGGGCAGGC GGCCCCCGGG 541 GCAGCTCCGA AACTGTGGGC GCAGCCCTCC TGCTCAACCT CCTTGCCGGC CTTGGAGCAA 601 TGGGGGCCAG GAGACCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGATGAGA ACCGAAAAGT 781 CCGTCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt